Inulinase from Aspergillus niger, CAS No.9025-67-6

CAS: 9025-67-6 Cat. No.: I755136 EC Number: 232-802-3 PubChem CID: 176259648

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, from Aspergillus Niger; ≥10000 U/g enzyme powder

Accession #

O74641,O74642

Bioactivity

≥10000 U/g enzyme powder

Overview

Inulinase (CAS No.9025-67-6, EC3.2.1.7) is derived from Aspergillus Niger which produced from submerged fermentation. It is mainly used to hydrolyze inulin into fructo-oligose, which can be widely used in many fields such as food, beverage and dairy products. 

Action Principle

This Inulinase is a endo-inulinase, which can hydrolyze β-2 , l-d- glycosidic bond of fructosan from inulin internal to form fructo-oligose. 

Product Characteristic

Optimum temperature: 50～60℃ 

Optimum pH: 4.5～5.5

Product Feature

Appearance: White to light brown powder, the colour may vary from batch to batch. 

Enzyme activity: ≥10000 U/g enzyme powder 

Enzyme activity definition: One inulinase unit is defined as the quantity of enzyme that will convert 1µmol reducing sugar per minute under the condition of hydrolyze the 5%(w/v) inulin 10 min at 60℃, pH 4.6. 

Product standard: GB1886.174-2024 <<National Food Safety Standard-Food Additives-Enzyme Preparations For Food Industry>>

Application:

In the 10% inulin solution, according to the quantity that add 400g inulinase into 1MT inulin, hydrolyze for 4 hours with the condition 55 ℃, pH4.5～5.0, it can get more than 95% fructo-oligose.  

Specifications

Product Name

Inulinase from Aspergillus niger, CAS No.9025-67-6

Synonyms

inulase | inuA | inuB

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, from Aspergillus Niger; ≥10000 U/g enzyme powder

Biochemical and Physiological Mechanisms

Endo-inulinase involved in utilization of the plant storage polymer inulin, consisting of fructooligosaccharides with a degree of polymerization (DP) value from 2 to 60.

Bioactivity

≥10000 U/g enzyme powder

Accession #

O74641, O74642

CAS

9025-67-6

Enzyme Commission Number

EC 3.2.1.7

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Aspergillus Niger; ≥10000 U/g enzyme powder

Reconstitution

Reconstitute in reverse osmosis water.

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Store in the dark. Desiccated.

Unit definition

One unit of inulinase activity is defined as the amount of enzyme required to produce 1 micromole of reducing sugar per minute when hydrolyzing a 5% (w/v) inulin solution for 10 minutes under the specified conditions (pH 4.6, 60 °C).