Mouse IgG1 ELISA Kit

Cat. No.: M1509874

GRADE & PURITY BioReagent

Overview

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of mouse IgG1 concentration in samples. The mouse IgG1 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, the mouse IgG1 present therein binds to the immobilized capture antibody. Unbound components are then removed through a washing step. Next, a horseradish peroxidase (HRP)-conjugated detection antibody specific for mouse IgG1 is added. This antibody binds to the captured mouse IgG1, forming a sandwiched immunocomplex. Following another wash to remove any unbound material, a colorimetric substrate solution (typically TMB) is added. In the presence of the immunocomplex, the conjugated HRP catalyzes the oxidation of the colorless substrate, producing a blue-colored product. The enzymatic reaction is terminated by adding a stop solution (usually an acid), which changes the color from blue to yellow. The absorbance, or Optical Density (OD), of each well is immediately measured at a wavelength of 450nm using a microplate reader.The concentration of mouse IgG1 in the sample is directly proportional to the OD450 value. A standard curve is constructed by plotting the OD450 values of known mouse IgG1 standard concentrations. The concentration of mouse IgG1 in unknown samples is then determined by interpolating their OD450 values from this standard curve.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months.