GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Phospholipase D (PLD, EC 3.1.4.4), also known as phosphatidylcholine hydrolase, is a general term for a class of enzymes that catalyze the hydrolysis of phosphodiester bonds and base‑exchange reactions. It is widely distributed in higher plants, animals, bacteria, and other organisms, and participates in various physiological processes including cellular lipid metabolism, signal transduction, biofilm formation, and stress resistance.
The assay principle of this kit is as follows: PLD catalyzes the hydrolysis of the terminal phosphodiester bond of phosphatidylcholine, generating phosphatidic acid and choline. Choline is then oxidized by choline oxidase to produce betaine and hydrogen peroxide (H₂O₂). In the presence of peroxidase (POD), H₂O₂ oxidizes 4‑aminoantipyrine and phenol to generate a pink quinone imine compound, which exhibits a characteristic absorption peak at 500 nm. By measuring the rate of change in absorbance at 500 nm, the PLD enzyme activity can be calculated.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
