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BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Xylanase (EC 3.2.1.8) is primarily produced by microorganisms and catalyzes the hydrolysis of xylan. It is also referred to as pentosanase or hemicellulase. This enzyme degrades cell wall components and β‑glucan in raw materials used in the brewing and feed industries, thereby reducing mash viscosity during brewing and facilitating the release of bioactive substances. In feed, it reduces non‑starch polysaccharides, enhancing nutrient digestibility and absorption. Consequently, it is widely applied in both brewing and feed production. Acid xylanase (ACX) is typically isolated from acid‑tolerant fungi, bacteria, and certain molds. Assay principle: Under acidic conditions, ACX hydrolyzes xylan into reducing oligosaccharides and monosaccharides. These reducing sugars react with 3,5‑dinitrosalicylic acid (DNS) in a boiling water bath, producing a colored compound with a characteristic absorption peak at 540 nm. The color intensity is directly proportional to the amount of reducing sugars generated. By measuring the rate of increase in absorbance at 540 nm, the ACX activity can be quantitatively determined.
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