Soil Acidic Xylanase Activity Assay Kit (DNS, Colorimetric Method)

Cat. No.: S1527403
AVAILABLE TO ORDER
GRADE & PURITY BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
Size
Status
Price
Qty
50T
S1527403-50T
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$107.90
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Why this grade

BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Xylanase (EC 3.2.1.8) is primarily produced by microorganisms and catalyzes the hydrolysis of xylan. It is also referred to as pentosanase or hemicellulase. This enzyme degrades cell wall components and β‑glucan in raw materials used in the brewing and feed industries, thereby reducing mash viscosity during brewing and facilitating the release of bioactive substances. In feed, it reduces non‑starch polysaccharides, enhancing nutrient digestibility and absorption. Consequently, it is widely applied in both brewing and feed production. Acid xylanase (ACX) is typically isolated from acid‑tolerant fungi, bacteria, and certain molds. Assay principle: Under acidic conditions, ACX hydrolyzes xylan into reducing oligosaccharides and monosaccharides. These reducing sugars react with 3,5‑dinitrosalicylic acid (DNS) in a boiling water bath, producing a colored compound with a characteristic absorption peak at 540 nm. The color intensity is directly proportional to the amount of reducing sugars generated. By measuring the rate of increase in absorbance at 540 nm, the ACX activity can be quantitatively determined.

Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

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🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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