Human B7-1/CD80 ELISA Kit

Cat. No.: H1509944

GRADE & PURITY BioReagent

Overview

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human B7-1/CD80 concentration in samples. The Human B7-1/CD80 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human B7-1/CD80 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human B7-1/CD80 is added. This antibody binds to the captured Human B7-1/CD80, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human B7-1/CD80 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman B7-1/CD80 is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman B7-1/CD80, and the concentration of Human B7-1/CD80 in unknown samples is interpolated from this curve based on their OD values.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months.