BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Mouse CCL11 Eotaxin concentration in samples. The Mouse CCL11 Eotaxin capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Mouse CCL11 Eotaxin present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Mouse CCL11 Eotaxin is added. This antibody binds to the captured Mouse CCL11 Eotaxin, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Mouse CCL11 Eotaxin is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofMouse CCL11 Eotaxin is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofMouse CCL11 Eotaxin, and the concentration of Mouse CCL11 Eotaxin in unknown samples is interpolated from this curve based on their OD values.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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