3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
This procedure is intended to standardize tissue pretreatment, fixation, sucrose cryoprotection, OCT embedding, rapid freezing, cryosectioning, section collection, frozen storage, and thawing procedures. It is applicable to immunofluorescence, immunohistochemistry, enzyme histochemistry, lipid ...
This protocol is used to establish a routine procedure for Helicobacter pylori May-Grünwald-Giemsa (MGG) staining. It is suitable for the morphological visualization of Helicobacter pylori in paraffin-embedded gastric mucosal tissue sections and can be used for auxiliary observation and ...
This protocol is used to establish the workflow for fixation, hydration, nuclear staining, cytoplasmic differential staining, dehydration, clearing, and mounting using the Papanicolaou EA36 system in cervical cytology specimens. Applicable samples include conventional cervical exfoliative cell ...
This protocol is used to determine phosphoglucose isomerase (PGI) activity in cell or microbial extracts. It is applicable to enzyme activity assays using microbial pellets from fermentation broth, cell lysate supernatants, and other soluble protein samples.
If the purpose of purification of the phage pellet is to obtain outgrown DNA fragments for subsequent subcloning or to prepare phage two-arms, centrifugation ca
DNA can be well isolated from large-scale preparations of λ phage by digestion with an effective protease (e.g., proteinase K) and subsequent phenol:chloroform
λ phage prepared from mass cultures can be recovered from the lysate by polyethylene glycolysis (PEG) precipitation in the presence of high salt. The residual
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