3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
This protocol is used to establish a routine workflow for G418 selection, single-clone isolation, clonal expansion, and stable cell line maintenance after CRISPR/Cas9 editing of mammalian cells. It is applicable to CRISPR/Cas9 vectors or co-transfection systems carrying the neomycin resistance ...
This protocol establishes an exosome extraction workflow based on TiO₂ magnetic beads. It is suitable for rapid enrichment, washing, and elution of exosomes from serum samples. The method is applicable to downstream research experiments such as exosome morphology observation, particle size ...
This protocol is intended to establish a routine decalcification workflow for mouse bone tissues. It is applicable to bone tissue pretreatment before paraffin sectioning and subsequent histological analysis. Depending on the downstream detection purpose, either the rapid decalcification method ...
This protocol is used to establish a standardized operating procedure for measuring alkaline phosphatase activity using a diethanolamine (DEA) buffer system. It is suitable for enzymatic determination of alkaline phosphatase products whose activities are calibrated using a diethanolamine assay ...
This protocol is used to establish a routine operating procedure for muscle fiber staining by the Puchtler tannic acid–azo phloxine method. It is suitable for differential observation of muscle fibers and collagen fibers in paraffin-embedded tissue sections. It can be used for morphological ...
Thomas Phosphomolybdic Acid Hematoxylin Method for Staining Collagen Fibers, Coarse Reticular Fibers, and Argyrophilic Cell Granules: Experimental Protocol
This protocol is used to establish a routine operating procedure for the Russell modified Movat pentachrome staining method. It is suitable for comprehensive color visualization of elastic fibers, collagen fibers, reticular fibers, proteoglycans, fibrinoid material, fibrin, and muscle tissue ...
Papanicolaou Staining Procedure
Feulgen Staining Procedure