Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...
The detection of cell surface proteins mainly targets antigens on the cell membrane. Antibodies can directly bind to antigens on the cell surface, so fixation and permeabilization treatment are usually not required. The experimental operation is relatively simple, and the main focus is on the ...
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