Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...
Topic
Antigen Retrieval
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Antigen retrieval in IHC determines the extent to which epitopes masked after fixation can be re-exposed, and it directly affects localization quality, signal intensity, and tissue integrity.
Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...
Related topics
Techniques often explored alongside immunological experiments.