Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...
After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
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