ChIP

Nucleic acid research spans confirmation of DNA/RNA presence, sequence-level characterization, and mechanistic dissection of interactions between nucleic acids and proteins or other nucleic acids. The choice of experimental method determines evidence type, resolution, throughput, and ...

ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).

Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...

This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP).

Detailed procedure and tips for cross-linking ChIP using ChIP-seq and ChIP-qPCR methods.