Topic

Immunoglobulin

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

10 resources · protocols, FAQs & articles
protocol

Multiplexed immunohistochemistry (mIHC) is an in situ multiple labeling method based on tyramide signal amplification (TSA) technology, which can achieve simultaneous detection of multiple targets (usually 6-8 types) on a single tissue section. Its core principle is to covalently bind the target ...

Updated Jun 2025·35 min read
protocol

Isotype control antibody is a negative control reagent that has the same immunoglobulin class, subclass, and light chain type as the research antibody (experimental antibody) but does not bind to the target antigen.

Updated Jun 2025·35 min read
article

Antibodies, also referred to as immunoglobulins (Ig), are significant Y-shaped glycoproteins produced by B-cells as a primary immune defense. These large proteins specially bind unique pathogen molecules that are known as antigens.

Updated Jun 2025·33 min read
Related topics

Techniques often explored alongside immunological experiments.

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