Goat Anti-Human IgG (AF555) - Secondary antibody, IgG

Cat. No.: Ab181961

GRADE & PURITY Validated, ExactAb™, Ex:553nm, Em:568nm, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

AF555

Descripción general

Goat Anti-Human IgG Antibody (AF555), Secondary antibody, could be used for IF, Flow

Specifications

Product Name

Goat Anti-Human IgG (AF555) - Secondary antibody, IgG

Sinónimos

Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I

Especificaciones y pureza

ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL

Especies hospedadoras

Goat

Conjugación

AF555

Excitación (nm)

Green Laser(532nm)

Emisión (nm)

568nm

Ex/Em(nm)

Ex:553nm;Em:568nm

Grado

Em:568nm, Ex:553nm, ExactAb™, Validated

Isotipo

IgG

Método de purificación

antigen affinity chromatography

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Imágenes

IF: Goat Anti-Human IgG (AF555) (Ab181961)

Goat Anti-Human IgG (AF555) (Ab181961) - IF
IF analysis of EGFR (red) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Modotuximab (anti-EGFR) (Ab182765) at 1 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF555) (Ab181961) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (AF555) (Ab181961)

Goat Anti-Human IgG (AF555) (Ab181961) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with Modotuximab (anti-EGFR) (Ab182765). Goat Anti-Human IgG (AF555) (Ab181961) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  1/200-1/2000;

• IF/ICC:

  1/100-1/1000 ;