Goat Anti-Human IgG (Cy5) - Secondary antibody

Cat. No.: Ab175833
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Ex:647nm ? Fluorescence excitation maximum at 647 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:665nm ? Fluorescence emission maximum at 665 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 1.0 mg/mL
Application
Flow Cytometry, IF/ICC
Host species
Goat
Reactive species
Human
Conjugation
Cy5
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
100μg
Ab175833-100μg
5
91,90US$
1mg
Ab175833-1mg
1
439,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm, 1.0 mg/mL Azide Free,Em:665nm,Ex:647nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application
Flow: 1:100-1:1000
IF: 1:1000

Specifications

Product Name
Goat Anti-Human IgG (Cy5) - Secondary antibody
Sinónimos
Goat Anti-Human Antibody (Cy5) | Cy5-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / Cy5 antibody | Goat Anti-Human IgG-Cy5 antibody
Especificaciones y pureza
ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm, 1.0 mg/mL
Especies hospedadoras
Goat
Conjugación
Cy5
Excitación (nm)
Red Laser(633nm)
Emisión (nm)
665nm
Ex/Em(nm)
Ex:647nm;Em:665nm
Grado
Azide Free, Em:665nm, Ex:647nm, ExactAb™, Validated
Método de purificación
Antigen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Imágenes

Goat Anti-Human IgG (Cy5) (Ab175833) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/100 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Human IgG (Cy5) (Ab175833) - IF
IF analysis of EGFR (red) in A549 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 2 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Aplicación
AplicaciónDilution info
IF/ICC

1:1000

Flow Cytometry

1/100-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeFechaArticulo
ZJ23F1001409Certificate of AnalysisMar 20, 2026 Ab175833
ZJ23F1001385Certificate of AnalysisFeb 10, 2026 Ab175833
Preguntas frecuentes y artículos
RNA dot blot protocol
Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies
Guidelines of Secondary Antibody for Western Blotting
Flow Cytometry Using Methanol Permeabilization
CLARITY Tissue Clearing Staining Technology
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:647nm grade guide → View Em:665nm grade guide →

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