IgG antihumana de cabra (AF405) - Secondary antibody, IgG

Cat. No.: Ab181959

GRADE & PURITY Validated, ExactAb™, Ex:401nm, Em:422nm, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

AF405

Descripción general

Aplicación
Caudal: 1:200-1:2000
IF: 1:100-1:1000.

Specifications

Product Name

IgG antihumana de cabra (AF405) - Secondary antibody, IgG

Sinónimos

Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana de cabra | Anticuerpo anti IgG humana

Especificaciones y pureza

ExactAb™, validado, Ex:401nm, Em:422nm, 1, 0 mg/mL

Especies hospedadoras

Goat

Conjugación

AF405

Excitación (nm)

Violet Laser(405nm)

Emisión (nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grado

Em:422nm, Ex:401nm, ExactAb™, Validated

Isotipo

IgG

Método de purificación

antigen affinity chromatography

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protegido de la luz,Conservar a -20°C,Evitar la congelación y descongelación repetidas

Enviado en

Hielera + almohadillas de hielo

Estabilidad y almacenamiento

Conservar a -20°C. Evitar el ciclo de congelación/descongelación. Proteger de la luz.

Imágenes

IF: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - IF
IF analysis of EGFR (blue) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Serclutamab (anti-EGFR) (Ab182867) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD276 (red) with Mirzotamab (anti-CD276) (Ab182952). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling IGF1R (red) with Ganitumab (anti-IGF1R) (Ab177931). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  1/200-1/2000;

• IF/ICC:

  1/100-1/1000 ;