Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG

Cat. No.: Ab181950
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:553nm ? Fluorescence excitation maximum at 553 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:568nm ? Fluorescence emission maximum at 568 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 1.0 mg/mL
Application
Flow Cytometry, IF/ICC
Host species
Goat
Reactive species
Mouse
Isotype
Goat IgG
Conjugation
AF555
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab181950-10μg
5
19,90US$
100μg
Ab181950-100μg
5
79,90US$
1mg
Ab181950-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
453,90US$
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL Em:568nm,Ex:553nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Goat Anti-Mouse IgG H&L Antibody, Polyclonal / Secondary antibody, could be uesd for Flow, IF.

Specifications

Product Name
Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
Sinónimos
Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I
Especificaciones y pureza
ExactAb™, Validated, Ex:553nm, Em:568nm, 1.0 mg/mL
Especies hospedadoras
Goat
Especificidad
Mouse IgG
Inmunógeno
Mouse IgG
Conjugación
AF555
Excitación (nm)
Green Laser(532nm)
Emisión (nm)
568nm
Ex/Em(nm)
Ex:553nm;Em:568nm
Grado
Em:568nm, Ex:553nm, ExactAb™, Validated
Isotipo
Goat IgG
Método de purificación
Antigen affinity chromatography
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Imágenes

Goat Anti-Mouse IgG H&L (AF555) (Ab181950) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 0.5 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF555) (Ab181950) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (AF555) (Ab181950) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (AF555) (Ab181950) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación
AplicaciónDilution info
Flow Cytometry

1:100-1:2000

IF/ICC

1/100-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0910378Certificate of AnalysisApr 17, 2026 Ab181950
ZJ24F0910379Certificate of AnalysisApr 17, 2026 Ab181950
ZJ24F0910380Certificate of AnalysisApr 17, 2026 Ab181950
Preguntas frecuentes y artículos
Citations of This Product
Referencias
1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:553nm grade guide → View Em:568nm grade guide →

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