Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody

Cat. No.: Ab156253

GRADE & PURITY Validated, ExactAb™, Azide Free, High Performance, Pre-adsorbed, 1.0 mg/mL

Application

ELISA, IF/ICC, WB

Host species

Goat

Reactive species

Mouse

Conjugation

Biotin

Descripción general

Application
WB: 1:10,000-1:200,000
ELISA: 1:20,000-1:400,000
IF: 1:1000

Specifications

Product Name

Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody

Sinónimos

Goat Anti-Mouse IgG H&L Antibody (Biotin) | Anti-Mouse IgG H&L Antibody (Biotin) | Mouse IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (Biotin) | Anti-Mouse IgG Antibody (Biotin) | Mouse IgG Antibod

Especificaciones y pureza

ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL

Especies hospedadoras

Goat

Inmunógeno

Mouse IgG

Control positivo

ELISA: Mouse IgG

Conjugación

Biotin

Grado

Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated

Método de purificación

Antigen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.

Imágenes

IF: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

ELISA: Goat Anti-Mouse IgG H&L (Biotin) (Ab156253)

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Aplicación

• Aplicación: Dilution info
• WB:

  1/10000-1/200000;

• IF/ICC:

  1:1000;

• ELISA:

  1/20000-1/400000;

Referencias

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]