Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG

Cat. No.: Ab179002

GRADE & PURITY Validated, ExactAb™, Azide Free, High Performance, 1.0 mg/mL

Application

ELISA, IF/ICC, WB

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

Biotin

Descripción general

Application
WB: 1:5,000-1:80,000
ELISA: 1:5,000-1:80,000

IF: 1:1,000  

Specifications

Product Name

Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG

Sinónimos

Goat Anti-Mouse IgG H&L Antibody (Biotin) | Anti-Mouse IgG H&L Antibody (Biotin) | Mouse IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (Biotin) | Anti-Mouse IgG Antibody (Biotin) | Mouse IgG Antibod

Especificaciones y pureza

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL

Especies hospedadoras

Goat

Inmunógeno

Mouse IgG

Conjugación

Biotin

Grado

Azide Free, ExactAb™, High Performance, Validated

Isotipo

Goat IgG

Método de purificación

Antigen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Imágenes

IF: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Western Blot: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at 1/20000 dilution
SA-HRP: 1:20000

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 67s

Flow Cytometry: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/300 was used as the secondary antibody. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: Goat Anti-Mouse IgG H&L (Biotin) (Ab179002)

Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1:1000;

• WB:

  1/5000-1/80000;

• ELISA:

  1:5000 - 1:80000 ;

Referencias

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]