Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, also known as Polyvinyl Alcohol Fluorescence Mounting Medium, is a mounting agent designed to slow down fluorescence quenching. It is also referred to as Fluorescence Signal-Enhancing Mounting Medium, and is similar to Sigma’s polyvinyl alcohol mounting medium containing DABCO (anti-fading type).
This medium can reduce the quenching effect of various common fluorescent dyes, with its anti-quenching performance significantly better than that of regular anti-quenching mounting media. Generally, the anti-quenching effect only begins to weaken after 2-3 weeks. Additionally, this mounting medium has the function of enhancing the fluorescence signal of rhodamine. Based on a dosage of 50-100 μl per sample, 5 ml of the reagent can be used for mounting at least 50 samples. This reagent is intended for research purposes only and not for clinical diagnosis or other applications.
Materials to Be Prepared by Users
Tissue samples, slides, coverslips, fluorescence microscope
Operating Procedures (For Reference Only)
1. Adherent Cell Samples
① After staining, aspirate the staining solution completely.
② Fully dissolve the Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, add 1 drop onto the slide, then place the coverslip with adherent cells onto the medium. Ensure the cells are in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 2-3 weeks.
2. Tissue Sections
① After staining, aspirate the staining solution completely.
② Fully dissolve the Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, add 1 drop onto the tissue section, then place a coverslip over it. Ensure the section is in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 2-3 weeks.
3. Other Samples
Follow the procedures for adherent cell samples or tissue sections.
Precautions
1. The concept of anti-fluorescence quenching is relative. Stained samples are best observed immediately after staining; if immediate observation is not feasible, observation should be completed on the same day.
2. All fluorescent substances are prone to quenching. Stained samples should be stored at 4 °C away from light.
3. Although this Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium can slow down quenching, samples should still be protected from light as much as possible during use and storage.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
ZJ26F0535668