GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
E. coli
Accession #
P01579
Protein Tag
No tag
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1. Recombinant human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells.The EC50 for this effect is typically 1.41 ng/mL. 2. Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
ActiBioPure™, Bioactive, Carrier Free, High Performance, PBS Only
Especificaciones y pureza
Carrier Free, Bioactive, ActiBioPure™, High Performance, PBS Only, ≥95%(SDS-PAGE)
Mecanismos bioquímicos y fisiológicos
IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymp
Bioactividad
1. Recombinant human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells.The EC50 for this effect is typically 1.41 ng/mL. 2. Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
14.1 kDa, under reducing conditions; 14.1 kDa, under non-reducing conditions
Tipo de molécula
Protein
Almacenamiento y envío
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Imágenes
Recombinant Human IFN-gamma Protein (rp186559) - Protein Bioactivity Recombinant Human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 1.41 ng/mL.
Recombinant Human IFN-gamma Protein (rp186559) - ELISA Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
Recombinant Human IFN-gamma Protein (rp186559) - SDS-PAGE 3 μg/lane of Recombinant Human IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.1 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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