Routine Glycerol Mounting Medium - BioReagent,for microscopy,Suitable for Immunofluorescence(IF),for fluorescence analysis

Cat. No.: C1508971

GRADE & PURITY BioReagent, for Fluorescence analysis, for Microscopy, Suitable for Immunofluorescence(IF)

Storage

Room temperature

Shipped In

Normal

Application

Cell Staining‌, IF/ICC

Descripción general

Product Introduction

Routine Glycerol Mounting Medium is a general-purpose mounting agent that can also be used to mitigate fluorescence quenching. It is mainly composed of glycerol. Its performance in reducing fluorescence quenching is inferior to that of Antifade Mounting Medium. Based on the dosage of 50–100 μl per sample, 5 ml of the medium can be used for mounting at least 50 samples. This reagent is intended for research use only and not for clinical diagnosis or other purposes.

Materials to Be Prepared by Users

Glass slides, coverslips, fluorescence microscope

Operating Procedures (For Reference Only)

1. Adherent cell samples

① After staining, aspirate the staining solution.

② Place 1 drop of Routine Glycerol Mounting Medium onto the glass slide, then cover it with the coverslip with adherent cells attached, ensuring the cells come into contact with the mounting medium and minimizing air bubbles as much as possible.

③ Observe under a microscope.

2. Tissue sections

① After staining, aspirate the staining solution.

② Place 1 drop of Routine Glycerol Mounting Medium onto the tissue section, then cover it with a coverslip, ensuring the section is in contact with the mounting medium and avoiding air bubbles as much as possible.

③ Observe the tissue section under a microscope.

3. Other samples

For other samples, follow the procedures for cell samples or tissue sections.

Precautions

1. All fluorescent substances are prone to quenching; stained samples should be stored away from light.

2. Although Routine Glycerol Mounting Medium can reduce quenching, samples mounted with it should still be protected from light as much as possible.

3. For your safety and health, wear a lab coat and disposable gloves during operation.

4. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.

Specifications

Especificaciones y pureza

BioReagent, for microscopy, Suitable for Immunofluorescence(IF), for fluorescence analysis

Estabilidad y almacenamiento

Store at room temperature long term (12 months).

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

BioReagent, for Fluorescence analysis, for Microscopy, Suitable for Immunofluorescence(IF)