BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Starch determination methods include acid hydrolysis and enzymatic analysis. Among these, the AACC 76-11 method employs autoclaving to gelatinize starch, followed by quantification after conversion to glucose using amyloglucosidase. However, this method tends to underestimate starch content in high-amylose maize starch and certain cereal products. This kit introduces an improvement by allowing thermostable α-amylase and amyloglucosidase to be incubated under the same pH conditions (pH 5.0), which simplifies the procedure and reduces the formation of by-products. For samples containing significant amounts of resistant starch, pre-dissolution in 1.7 M KOH or hot DMSO is recommended before enzymatic treatment. The assay principle is as follows: Thermostable α-amylase hydrolyzes starch to maltodextrins, which are then quantitatively hydrolyzed to D-glucose by amyloglucosidase. The D-glucose is oxidized by glucose oxidase to generate H₂O₂. In the presence of peroxidase, the H₂O₂ reacts with a chromogenic substrate to form a quinoneimine dye. The absorbance of this dye is measured colorimetrically for quantification.
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