Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3

Cat. No.: Ab156544
주문 가능
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, IF/ICC, WB
Host species
마우스
Reactive species
마우스, 인간, 조언
Isotype
Mouse IgG2a
Clone Number
CD01/2F2
Conjugation
Unconjugated
★
Size
USA
독일 (EU)*
Price
Qty
10μl
Ab156544-10μl
9 재고 있음
—
US$99.90
50μl
Ab156544-50μl
1 재고 있음
—
US$259.90
100μl
Ab156544-100μl
1 재고 있음
—
US$419.90
1ml
Ab156544-1ml
US 주문제작 · 2–4주 ·
—
US$3,399.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

Mouse anti Human CASP3 Antibody, clone CD01/2F2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name
Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3
동의어
A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3 | Caspase-3 subunit p12 antibody | Caspase3 antibody | CC3 antibody | CPP 32 antibody
사양 및 순도
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
생화학 및 생리적 메커니즘
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many protein
숙주 종
마우스
특이성
Human CASP3
면역원
Recombinant protein corresponding to Human CASP3
포지티브 제어
WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver tissue lysates Flow Cytometry: Jurkat cells IF/ICC: HeLa cells
변화
Unconjugated
등급
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
라이트 체인 유형
kappa
SDS-PAGE
150.0 KDa
정화 방법
Protein A purified
집중력
1.0 mg/mL
보관 및 배송
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
이미지

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34,36 kDa
Exposure time: 16.2 s 

Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s 

Caspase 3 Mouse mAb (Ab156544) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with Caspase 3  Mouse mAb (Ab156544) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue). ​

Caspase 3 Mouse mAb (Ab156544) - ICC/IF
IF analysis of Caspase 3 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase 3 Mouse mAb (Ab156544) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

적용
적용
Dilution info
IF/ICC

1/200

WB

1/1000 - 1/200000

Flow Cytometry

1/200 - 1/12500

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
CASP3 Tchem Caspase-3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type날짜항목
ZJ25F0116463Certificate of AnalysisOct 09, 2026 Ab156544
ZJ25F0116464Certificate of AnalysisOct 09, 2026 Ab156544
ZJ25F0116465Certificate of AnalysisOct 09, 2026 Ab156544
자주 묻는 질문과 기사
솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

관련 상품

Goat Anti-Mouse IgG H&L Antibody - 2차 항체, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - 아이소타입 대조 항체, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - 2차 항체, IgG
BALB/c Mouse IgG - 아이소타입 대조 항체, IgG
Recombinant Caspase3 Antibody - 1차 항체, specific to CASP3, Rabbit IgG
Rabbit Anti-Mouse IgG antibody - 2차 항체
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체
Goat Anti-Mouse IgG H&L Antibody - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체
Rabbit Anti-Mouse IgG (HRP) - 2차 항체
Rabbit Anti-Mouse IgG (Biotin) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 2차 항체, Goat IgG
Mouse IgG (Biotin) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (PE) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (APC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (FITC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG2a-Fc - 아이소타입 대조 항체, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 2차 항체, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - 아이소타입 대조 항체, IgG
Mouse IgG (PerCP) - 아이소타입 대조 항체, IgG
Mouse IgG (AF405) - 아이소타입 대조 항체, IgG
Mouse IgG (AF647) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF555) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF700) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - 아이소타입 대조 항체
Mouse IgG (PE-Cy7) - 아이소타입 대조 항체
마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L
마우스 IgG2a-카파, 항-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - 아이소타입 대조 항체
Mouse IgG2a-Fc (FITC) - 아이소타입 대조 항체
Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3
Mouse IgG (AF555) - 아이소타입 대조 항체
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - 2차 항체, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 1차 항체, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - 2차 항체
Goat Anti-Mouse IgG H&L (PE-Cy7) - 2차 항체
Mouse IgG (PE-Cy5.5) - 아이소타입 대조 항체
Goat Anti-Mouse IgG H&L (AF750) - 2차 항체
Mouse IgG2a-kappa (AF555), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - 아이소타입 대조 항체, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - 2차 항체
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 2차 항체
Goat Anti-Mouse IgG H&L (Cy3) - 2차 항체
Mouse IgG2a-kappa (APC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG (PE-AF647) - 아이소타입 대조 항체

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.