Recombinant Caspase3 Antibody - Primary antibody, specific to CASP3, Rabbit IgG

Cat. No.: Ab093013
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.9mg/mL
Application
IF/ICC, IHC, WB
Host species
Rabbit
Reactive species
Human
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
50μl
Ab093013-50μl
2
$207.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.9mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human Caspase3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Specifications

Product Name
Recombinant Caspase3 Antibody - Primary antibody, specific to CASP3, Rabbit IgG
Synonyms
A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3, apoptosis-related cysteine peptidase antibody | Caspase 3, apoptosis-related cysteine
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.9mg/mL
Host species
Rabbit
Specificity
CASP3
Immunogen
A synthetic peptide derived from human Caspase-3 (AA 75-94).
Positive Control
WB: Jurkat, HeLa, THP-1, Hep G2 and K562 cell lysates; IHC: Human bladder cancer tissues; IF: 293
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
0.9mg/mL
Note
This product is about to be sold out and discontinued. The replacement product is Ab093009
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant Caspase3 Antibody (Ab093013) - Western Blot
All lanes: Recombinant Caspase3 Antibody (Ab093013) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 32 kDa
Observed band size: 30 kDa
Exposure time: 1.0 s

Recombinant Caspase3 Antibody (Ab093013) - ICC/IF
IF analysis of HEK293 cells using Recombinant Caspase3 Antibody (Ab093013) (green),and cells were counterstained with DAPI (blue).

Recombinant Caspase3 Antibody (Ab093013) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Recombinant Caspase3 Antibody (Ab093013). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CASP3 Tchem Caspase-3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CASP3 Tchem Caspase-3 (3632 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800785Certificate of AnalysisAug 14, 2023 Ab093013
ZJ23F0800790Certificate of AnalysisAug 14, 2023 Ab093013
Documents & Articles
How to choose and use antibodies-primary antibody?
Antibody applications and techniques
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
Caspase Immunofluorescence Staining Protocol
Apoptosis Induction Methods
Molecular Composition, Signal Transduction Mechanisms, and Tissue Injury Regulation of the TWEAK-Fn14 Pathway
TNF Signaling Pathway: Receptor Layering Structure, Complex Conversion Mechanisms, and the Inflammation–Death Effector Network
Killer T Cell-Mediated Apoptosis Pathway: Recognition, Effector Molecule Release, and Target Cell Death Mechanisms
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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