Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3

Cat. No.: Ab213595
주문 가능
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, WB
Host species
마우스
Reactive species
마우스, 인간, 조언
Isotype
Mouse IgA
Clone Number
CD04/3D12
Conjugation
Unconjugated
★
Size
USA
독일 (EU)*
Price
Qty
10μl
Ab213595-10μl
5 재고 있음
—
US$99.90
50μl
Ab213595-50μl
재고 있음 ≥10
—
US$259.90
100μl
Ab213595-100μl
3 재고 있음
—
US$419.90
1ml
Ab213595-1ml
US 주문제작 · 2–4주 ·
—
US$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

Mouse anti Human CASP3 Antibody, clone CD04/3D12, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name
Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3
동의어
A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3 | Caspase-3 subunit p12 antibody | Caspase3 antibody | CC3 antibody | CPP 32 antibody
사양 및 순도
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
생화학 및 생리적 메커니즘
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many protein
숙주 종
마우스
특이성
Human CASP3
면역원
Recombinant protein corresponding to Human CASP3
포지티브 제어
WB: Jurkat, HeLa, HEK293, NIH/3T3 and C6 whole cell lysates; Mouse liver and Mouse lung tissue lysates Flow Cytometry: Jurkat cells
변화
Unconjugated
등급
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
라이트 체인 유형
lambda
SDS-PAGE
150.0 KDa
정화 방법
Protein A purified
집중력
1.0 mg/mL
보관 및 배송
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
이미지

Caspase 3 Mouse mAb (Ab213595) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab213595) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 31,34 kDa
Exposure time: 60.0 s

Caspase 3 Mouse mAb (Ab213595) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab213595) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 33 kDa
Exposure time: 60.0 s

aspase 3 Mouse mAb (Ab213595) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with Caspase 3  Mouse mAb (Ab213595) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

적용
적용Dilution info
WB

1/1000 - 1/200000

Flow Cytometry

1/200 - 1/12500

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
CASP3 Tchem Caspase-3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
자주 묻는 질문과 기사
Flow Cytometry Using Methanol Permeabilization
Molecular Composition, Signal Transduction Mechanisms, and Tissue Injury Regulation of the TWEAK-Fn14 Pathway
TNF Signaling Pathway: Receptor Layering Structure, Complex Conversion Mechanisms, and the Inflammation–Death Effector Network
Killer T Cell-Mediated Apoptosis Pathway: Recognition, Effector Molecule Release, and Target Cell Death Mechanisms
Proteolytic Enzyme Networks in Cell Injury: Activation Sequence, Substrate Cleavage, and Experimental Attribution
Applications of the PLSCR Family in Cell Death, Coagulation, and Inflammatory Signaling Research
솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

관련 상품

Goat Anti-Mouse IgG H&L Antibody - 2차 항체, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - 아이소타입 대조 항체, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - 2차 항체, IgG
BALB/c Mouse IgG - 아이소타입 대조 항체, IgG
Recombinant Caspase3 Antibody - 1차 항체, specific to CASP3, Rabbit IgG
Rabbit Anti-Mouse IgG antibody - 2차 항체
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체
Goat Anti-Mouse IgG H&L Antibody - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체
Rabbit Anti-Mouse IgG (HRP) - 2차 항체
Rabbit Anti-Mouse IgG (Biotin) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 2차 항체, Goat IgG
Mouse IgG (Biotin) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (PE) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (APC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG (FITC) - 아이소타입 대조 항체, Mouse IgG
Mouse IgG2a-Fc - 아이소타입 대조 항체, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 2차 항체, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - 아이소타입 대조 항체, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - 아이소타입 대조 항체, IgG
Mouse IgG (PerCP) - 아이소타입 대조 항체, IgG
Mouse IgG (AF405) - 아이소타입 대조 항체, IgG
Mouse IgG (AF647) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF555) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - 아이소타입 대조 항체, IgG
Goat Anti-Mouse IgG H&L (AF700) - 2차 항체, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - 아이소타입 대조 항체
Mouse IgG (PE-Cy7) - 아이소타입 대조 항체
마우스 IgG2b-카파, 항-E8L - 아이소타입 대조 항체, specific to E8L
마우스 IgG2a-카파, 항-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - 아이소타입 대조 항체
Mouse IgG2a-Fc (FITC) - 아이소타입 대조 항체
Caspase 3 Mouse mAb - 1차 항체, specific to Human CASP3
Mouse IgG (AF555) - 아이소타입 대조 항체
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - 2차 항체, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 1차 항체, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - 아이소타입 대조 항체, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - 2차 항체
Goat Anti-Mouse IgG H&L (PE-Cy7) - 2차 항체
Mouse IgG (PE-Cy5.5) - 아이소타입 대조 항체
Goat Anti-Mouse IgG H&L (AF750) - 2차 항체
Mouse IgG2a-kappa (AF555), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - 아이소타입 대조 항체, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - 2차 항체
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 2차 항체
Goat Anti-Mouse IgG H&L (Cy3) - 2차 항체
Mouse IgG2a-kappa (APC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG (PE-AF647) - 아이소타입 대조 항체

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