GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Citrate synthase (CS, EC 2.3.3.1) is widely distributed in the mitochondrial matrix of animals, plants, microorganisms, and cultured cells. It is the first rate-limiting enzyme of the tricarboxylic acid (TCA) cycle and one of the major regulatory sites of this cycle.
The assay principle of this kit is as follows: CS catalyzes the condensation of acetyl-CoA and oxaloacetate to form citryl-CoA, which is subsequently hydrolyzed to produce citrate. Concurrently, this reaction drives the conversion of colorless DTNB to yellow TNB, which exhibits a characteristic absorption peak at 412 nm. By measuring the rate of increase in absorbance at 412 nm, the enzyme activity of CS can be calculated.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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