Preparation of pseudotyped lentiviral vectors resistant to complement inactivation
This experimental protocol describes several methods for generating complement-stabilized lentiviral vectors either by using fusion packaging proteins containin
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Lentiviral transduction of hematopoietic cells
Most hematopoietic cells, including hematopoietic stem cells and terminally differentiated cells such as primary T cells and macrophages, are undifferentiated o
Reverse transcription virus vector assay
Reverse transcription viruses are well suited to be used as gene introduction tools because of their specific life cycle and ability to integrate efficiently in
Construction of first-generation adenoviral vectors
The possibility of immune responses against proteins encoded by viral vector backbones limits the application of transgene vectors for in vivo expression. Never
technology that targets genes
Gene pooping is an experimental tool for targeted alteration of genetic information of a species based on embryonic stem cells (ESCs) and homologous recombinati
Design method for adeno-associated heterotrimeric viral vectors
A A V coat peptide display library, error-prone P C R and D N A recombination . A A V serotypes with different histophilicity can be generated by natural mutati
Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
Polylysine copolymers for gene delivery
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p
Gene gun technology for delivering genes into the skin
Gene gun technology is a fast and simple technique for delivering genes into cells. This technology has many advantages: it allows direct delivery of plasmids w