Reverse genetics of influenza viruses
It mainly describes the methods used to recover infectious influenza viruses from cDNA clones. These techniques are widely used in the integration and expressio
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3228 articles
Gene gun technology for delivering genes into the skin
Gene gun technology is a fast and simple technique for delivering genes into cells. This technology has many advantages: it allows direct delivery of plasmids w
Preservation and amplification of targeted modified measles viruses
The S T A R System (6 Histidine Labeling and Retargeting) employs a pseudo-receptor and a label for a virus to enable the rescue and amplification of fully reco
Recombinant herpes simplex virus vector
The natural physiological characteristics of H S V -I include the establishment of a lifelong latent state in neurons, where the viral gene remains a free molec
Site-specific integration experiments mediated by bacteriophage ΦC31 integrase
Efficient delivery of ΦC31 integrase and donor plasmids to recipient tissues or cells of interest remains the most challenging element of this integrative syst
Down-regulation of genes generated by silencing lentiviral vectors in mice.
This chapter describes the use of lentiviral vectors to transfer genes and small interfering RNA (siR N A ) expression cassettes to preimplantation crystalline
Experiments on the isolation of subcellular organelles and subcellular structures
In proteomics research, a key step in all enrichment techniques is the subcellular fractionation, which is particularly important for the analysis of intracellu
Experiments with biodegradable nanoparticles
Biodegradable nanoparticles are colloidal particles, typically around IOOnm in diameter, which can be prepared from biodegradable polymers such as polylactic ac
Non-viral gene transfer experiments with Trojan horse liposomes that can cross the blood-brain barrier
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula