Experiments on Prokaryotic Microinjection Technique
A technique for preparing transgenic mice by prokaryotic microinjection is described. Prokaryotic microinjection, which involves the injection of DNA into haplo
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3228 articles
Site-specific integration experiments mediated by bacteriophage ΦC31 integrase
Efficient delivery of ΦC31 integrase and donor plasmids to recipient tissues or cells of interest remains the most challenging element of this integrative syst
Experiments on the theory and application of hydrophobic interaction chromatography in protein purification
Hydrophobic interaction chromatography (HIC) is a valuable tool in protein purification technology. HIC is used for protein purification on a wide range of scal
Separation techniques of oxygen-sensitive proteins: [4Fe-4S]-FNR as an example
Describes the methods used to isolate the oxygen-sensitive iron-sulfur protein FNR in the laboratory. Authors: Burgess et al., Translator: Chen Wei, this experi
Parallel method experiments for expression and purification
Proteins are diverse in nature, which makes it challenging to express and purify proteins using parallel methods. Parallel methods can especially be applied whe
Experiments to determine the presence of protein subunits and their size and molecular mass
The size or apparent atomic mass of proteins is perhaps the most common way of distinguishing the nature of a molecule. As a basis for many separation methods,
Cell-free translation experiments of integral membrane proteins in single-compartment liposomes
Membrane proteins provide the appropriate molecular mechanisms that allow the controlled entry of useful molecules into the cell and permit the export of intrac
One-way gel electrophoresis experiment
The S D S -- P A G E methods presented in this book will fulfill the needs of most applications in this field. Authors: Burgess et al, Translator: Chen Wei, Thi
Immobilized metal affinity chromatography experiments
Summarizes the application areas of IMAC, including protein hierarchical separation and proteomics, protein fixation and detection, as well as some special appl