GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Acetolactate synthase (ALS, EC 2.2.1.6) is a key enzyme in the biosynthesis of branched‑chain amino acids. It is present exclusively in plants and microorganisms, making it an important target for green herbicides. This kit employs a colorimetric method for the determination of ALS activity. The principle is as follows: ALS catalyzes the formation of acetolactate from pyruvate. The acetolactate is then decarboxylated to acetoin by treatment with sulfuric acid, which subsequently reacts with a chromogenic reagent to produce a colored product with a characteristic absorption peak at 525 nm. ALS activity can be calculated by measuring the rate of formation of this product.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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