GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
The quantitative determination of β-glucan is based on a series of coupled enzymatic reactions: first, β-glucan is hydrolyzed by lichenase to oligosaccharides, which are then completely degraded to glucose by β-glucosidase. Glucose is oxidized by glucose oxidase to gluconic acid, with concomitant production of H₂O₂. Subsequently, in the presence of peroxidase, H₂O₂ reacts with 4-aminoantipyrine and phenolic derivatives to form a purple compound, which exhibits a maximum absorbance peak at 540 nm. Therefore, by measuring the change in absorbance of the reaction system at 540 nm, the β-glucan content can be accurately determined.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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