(4R-cis)-5-methyl-2-oxoimidazolidine-4-hexanoic acid - ≥95% , CAS No.533-48-2

CAS: 533-48-2 Cat. No.: R303913 Molecular Weight: 214.26 EC Number: 208-566-2 PubChem CID: 445027

GRADE & PURITY ≥95%

Synonyms

(+)-Dethiobiotin | (4R,5S)-dethiobiotin | Desthiobiotin, (+/-)- | (+/-)-Desthiobiotin | dl-Dethiobiotin | Desthiobiotin | d-Dethiobiotin | d-Desthiobiotin|dethiobiotin | 6-[(4R,5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanoic acid

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

D-desthiobiotin has been used in affinity chromatography and protein chromatography.D-desthiobiotin can be used for protein labeling, detection, and separation.

Product Application：
d-Desthiobiotin has been used as a component of elution buffers:
to elute the precipitated proteins from the resin during coimmunoprecipitation
to elute human Cdc45–MCM–GINS (CMG) helicase during chromatographic protein purification
to elute the heterodimeric kinesin family member 3A/B (KIF3A/B) and kinesin associated protein 3 (KAP3)-binding APC armadillo (APCARM) domain during chromatographic purification
d-Desthiobiotin is used in affinity chromatography and protein chromatography. d-Desthiobiotin can be used for protein labeling, detection and isolation.

Specifications

Synonyms

(+)-Dethiobiotin | (4R, 5S)-dethiobiotin | Desthiobiotin, (+/-)- | (+/-)-Desthiobiotin | dl-Dethiobiotin | Desthiobiotin | d-Dethiobiotin | d-Desthiobiotin | dethiobiotin | 6-[(4R, 5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanoic acid

Specifications & Purity

≥95%

Biochemical and Physiological Mechanisms

Desthiobiotin is a non-sulfur containing biotin derivative that binds less strongly to biotin-binding proteins and is easily dislocated by biotin.

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%

Names and Identifiers

• Pubchem Sid: 504758835
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504758835
• Canonical Smiles: CC1C(NC(=O)N1)CCCCCC(=O)O
• IUPAC Name: 6-[(4R,5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanoic acid
• InChIKey: AUTOLBMXDDTRRT-JGVFFNPUSA-N
• INCHI: 1S/C10H18N2O3/c1-7-8(12-10(15)11-7)5-3-2-4-6-9(13)14/h7-8H,2-6H2,1H3,(H,13,14)(H2,11,12,15)/t7-,8+/m0/s1
• Isomeric SMILES: C[C@H]1[C@H](NC(=O)N1)CCCCCC(=O)O
• PubChem CID: 445027
• Molecular Weight: 214.26

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Lipids and lipid-like molecules
    • Class Fatty Acyls
      • Subclass Fatty acids and conjugates

• Kingdom: Organic compounds
• Superclass: Lipids and lipid-like molecules
• Class: Fatty Acyls
• Subclass: Fatty acids and conjugates
• Intermediate Tree Nodes: Not available
• Direct Parent: Medium-chain fatty acids
• Alternative Parents: Heterocyclic fatty acids Amino fatty acids Imidazolidinones Ureas Monocarboxylic acids and derivatives Carboxylic acids Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic heteromonocyclic compounds
• Substituents: Medium-chain fatty acid - Amino fatty acid - Heterocyclic fatty acid - Imidazolidinone - Imidazolidine - Carbonic acid derivative - Urea - Carboxylic acid derivative - Carboxylic acid - Monocarboxylic acid or derivatives - Azacycle - Organoheterocyclic compound - Carbonyl group - Organic oxide - Organopnictogen compound - Organic oxygen compound - Hydrocarbon derivative - Organonitrogen compound - Organooxygen compound - Organic nitrogen compound - Aliphatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as medium-chain fatty acids. These are fatty acids with an aliphatic tail that contains between 4 and 12 carbon atoms.
• External Descriptors: dethiobiotin

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

HEK293 (82097 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

NR2E3 Tchem Photoreceptor-specific nuclear receptor (502 Activities)

Discover Target: NR2E3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

POLH Tchem DNA polymerase eta (21678 Activities)

Discover Target: POLH

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

KDM4A Tchem Lysine-specific demethylase 4A (52245 Activities)

Discover Target: KDM4A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BLM Tchem Bloom syndrome protein (4248 Activities)

Discover Target: BLM

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)

Discover Target: SMAD3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GMNN Tbio Geminin (128009 Activities)

Discover Target: GMNN

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

IDE Tchem Insulin-degrading enzyme (806 Activities)

Discover Target: IDE

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CBX1 Tbio Chromobox protein homolog 1 (92434 Activities)

Discover Target: CBX1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (40980 Activities)

Discover Target: IDH1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PPARG Tclin Peroxisome proliferator-activated receptor gamma/Nuclear receptor corepressor 2 (239 Activities)

Discover Target: PPARG

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

WRN Tbio Werner syndrome ATP-dependent helicase (8824 Activities)

Discover Target: WRN

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

NS1 Nonstructural protein 1 (33327 Activities)

Discover Target: NS1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Flash Point(°C): 241.9 °C
• Boil Point(°C): 476.4 °C at 760 mmHg
• Melt Point(°C): 156-158 °C
• Molecular Weight: 214.260 g/mol
• XLogP3: 0.800
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 6
• Exact Mass: 214.132 Da
• Monoisotopic Mass: 214.132 Da
• Topological Polar Surface Area: 78.400 Ų
• Heavy Atom Count: 15
• Formal Charge: 0
• Complexity: 243.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 2
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Xingdong Dong, Qi Lu, Yiping Zhang, Limin Yang.  (2025)  Microfluidic magnetic solid-phase extraction of acetamiprid using dynamically manipulated apta-magnetic sorbents for chromatographic analysis.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:40816227] [10.1016/j.chroma.2025.466273]

2. Chi Wang, Fahui Sun, Heyu Zhao, Nan Zhang, Jiali Guan, Yuxing Zhou, Wentong Shuai, Hui Zheng, Jun He.  (2026)  Structural Basis of Nucleosome Deubiquitination by the Bidentate Calypso/Asx Complex.  iScience,      [PMID:41782825] [10.1016/j.isci.2026.114958]