GRADE & PURITYMoligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
This compound belongs to the class of organic compounds known as alpha amino acids and derivatives. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon), or a derivative thereof.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
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Application Protocols
No tested applications or recommended protocols are provided for ABL127 in the Product Data (e.g., no WB/IHC/IF/FC guidance, no enzymology protocol). As a Moligand-class small molecule, typical usage is user-defined based on the intended assay.
General protocol template for small-molecule screening (not item-specific):
Stock: Prepare 10–50 mM in anhydrous DMSO. Aliquot and store sealed to avoid moisture uptake.
Serial dilutions: Perform in DMSO or assay buffer with constant DMSO percentage across wells. Aim for 10‑point 3‑fold or 1:2 series for robust curve fitting.
Controls: Include vehicle control (DMSO-only), positive control (if available), and non-specific control to check for interference.
Readout validation: Check for compound-related assay interference (intrinsic fluorescence/absorbance, redox activity, aggregation) with appropriate counterscreens.
Users should adapt these steps to their assay modality and consult the CoA/SDS prior to use.
Biological Roles
Item-specific biological targets, pathways, and roles are not provided for ABL127 in the Product Data. Therefore, no definitive claims can be made for this item’s mode of action, selectivity, or cellular effects.
General considerations for small-molecule library members (not item-specific):
Such compounds are commonly used as tool molecules in exploratory studies to modulate enzymes, receptors, transporters, or signaling pathways. Verification of on-target effects typically requires orthogonal assays (e.g., biochemical potency, cellular phenotypes, chemoproteomics/thermal shift).
Off-target assessment may include panel screening, counterscreens for assay artifacts (fluorescence/quenching, redox cycling), and cytotoxicity profiling.
For cellular work, evaluate permeability, efflux liability, and metabolic stability (e.g., microsomes/S9) to contextualize observed phenotypes.
Recommendation: Consult the CoA/Spec Sheet and primary literature corresponding to the provided PubChem CID for putative biological roles, then confirm experimentally under your laboratory conditions. This product is for research use only.
Buffer Applications
Not typically applicable. ABL127 is offered as a small-molecule library member rather than a buffering reagent.
Practical notes for assay buffers (general guidance):
If using the compound in biochemical or cell-based assays, dissolve first in DMSO (or another appropriate organic solvent), then dilute into the working buffer (e.g., PBS, HEPES, Tris) to the desired final concentration while keeping organic cosolvent low (≤0.1–1% v/v).
Confirm solubility and absence of precipitation in the chosen buffer by visual inspection and by LC/UV after incubation at assay temperature.
Green Alternatives
For small molecules used primarily as assay ligands rather than bulk reagents or solvents, “green alternatives” considerations mainly concern solvent and workflow choices rather than substituting the compound itself.
Prefer DMSO for stock solutions over chlorinated solvents; it combines high solvency with acceptable toxicity and environmental profile in small volumes.
When feasible, consider greener dipolar aprotics (e.g., Cyrene or propylene carbonate) for preparative workups or analytics, after confirming compound stability and assay compatibility.
Minimize solvent volumes via higher-concentration stocks and miniaturized assays (HTS-friendly 384/1536 plates) to reduce waste.
Trade-offs (general):
Cyrene/propylene carbonate have higher viscosity and different polarity than DMF/DMAC/NMP and may not dissolve very lipophilic compounds as effectively.
DMSO, while broadly accepted, is hygroscopic and can transport compounds through skin—maintain strict PPE and handling discipline.
Waste reduction:
Consolidate and segregate DMSO- and acetonitrile-rich wastes for optimized disposal.
Use sealed, low-dead-volume reservoirs and plate lids/seals to limit evaporation and VOC emissions.
Item-specific greener substitutes for ABL127 are not applicable because the active chemical identity and function are not specified in the Product Data.
Pharmaceutical Uses
No pharmacopeial status, excipient role, or manufacturing use is provided for ABL127. This product is supplied as a research-only small molecule (Moligand grade) and is not intended for human or veterinary use, nor for clinical or GMP manufacturing.
General information (not item-specific):
Tool compounds from discovery libraries are sometimes used in formulation-relevant studies (e.g., permeability models, analytical method development) but are not validated as pharmaceutical excipients or APIs.
If formulation-like studies are needed (e.g., solubility enhancement, stability in vehicles), conduct them under research protocols and do not construe results as suitable for clinical translation.
Please refer to the CoA/Spec Sheet for quality tests relevant to research use and consult appropriate pharmacopeial monographs only if a separate, compendial-grade material is procured.
Physical Properties
The following item-specific physicochemical parameters are not provided in the Product Data and should be obtained from the CoA/Spec Sheet for the specific lot you receive:
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
Density: Not specified for this item; refer to CoA/Spec Sheet.
Solubility profile (water/organic): Not specified for this item; refer to CoA/Spec Sheet.
LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
pKa: Not specified for this item; refer to CoA/Spec Sheet.
Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
General guidance for small-molecule library members (literature/practice, not item-specific):
Many bioactive screening compounds are moderately to highly lipophilic and are most reliably prepared as DMSO stock solutions (e.g., 10–50 mM), then diluted into assay buffer to ≤1% v/v DMSO final.
If aqueous use is intended, perform a mini solubility screen (DMSO, DMF, ethanol, methanol, PBS with cosolvent or surfactant) and confirm by visual inspection and LC/UV.
Hygroscopicity and polymorphism can affect apparent MP and dissolution rates; verify lot-specific data on receipt.
Quality & Grades
Grade/Purity: Moligand™ (as provided)
What Moligand™ typically denotes (general description; consult product page/CoA for this item’s release tests):
A screening-grade small-molecule ligand intended for biochemical/cellular assays, target engagement studies, and library applications.
Emphasis on identity confirmation and suitability for hit finding or MOA exploration rather than chromatographic solvent grade or pharmacopoeial compliance.
Practical implications for this item:
Identity/purity: Lot-specific confirmation by techniques such as HPLC/UPLC and MS/HRMS is typical for Moligand-class offerings. Exact test set and acceptance criteria are defined on the CoA/Spec Sheet for A607328.
Stabilizers/inhibitors: Not specified for this item; refer to CoA/Spec Sheet.
Absorbance background/UV cutoffs: Not specified for this item; refer to CoA/Spec Sheet. If using in optical assays, verify baseline spectra of your lot in the working solvent.
Suitability: Appropriate for discovery research, method development, and preliminary SAR. For regulated uses or QC analytics requiring compendial status, this grade is not intended.
Recommendation: Upon receipt, document lot number and retain CoA. If your assays are stability-sensitive, perform a quick integrity check (HPLC purity and MS) after solution preparation.
Reaction & Applications
This product is positioned as a small-molecule/compound-library member for research use. No manufacturer application notes are provided for ABL127. Accordingly, the following are general, non–item-specific research uses of Moligand-class small molecules:
Target engagement and MOA studies: Use in biochemical/cellular assays to probe enzyme or receptor pathways, typically via concentration–response curves to derive potency metrics (IC50/EC50) where applicable.
Chemical biology workflows: Suitable for pilot chemoproteomics, phenotypic screening, or pathway mapping, provided orthogonal controls are included.
Assay development: Useful as a putative positive control or tool compound in method development after in-house validation.
Practical tips (general):
Prepare fresh DMSO stocks at known concentration (e.g., by weight with density correction or by quantitative NMR) and aliquot to avoid freeze–thaw.
Confirm integrity by LC/MS after dilution into assay buffer (look for precipitation or degradation peaks).
Include vehicle controls and, where possible, reference standards to contextualize activity.
Note: Because no structural or mechanistic specifics are supplied for this item, do not assume reactivity or biological mechanism without confirming against the CoA and your own validation data.
Reaction Conditions
Not applicable in a synthetic sense: ABL127 is supplied as a small-molecule tool compound, not as a reagent or catalyst with defined reaction conditions.
General handling for solution preparation (assay context; not item-specific):
Stock preparation: Dissolve in dry DMSO to 10–50 mM. Vortex and, if needed, sonicate briefly to aid dissolution. Record exact concentration by weight-in-volume or by quantitative 1H NMR if precision is required.
Working solutions: Dilute into assay medium to target concentration, maintaining final DMSO at levels compatible with your system (commonly ≤0.1–1% v/v). Mix thoroughly to avoid local supersaturation.
Stability checks: Incubate representative samples at assay temperature and analyze by LC/MS to confirm no rapid degradation over the assay window.
If you intend to use this compound as a reagent in chemical synthesis, obtain full structural information from the CoA and consult primary literature for conditions specific to its functional groups.
Safety & Handling
Hazard information (as provided):
Signal word: Not specified for this item; refer to SDS.
H‑statements: Not specified for this item; refer to SDS.
GHS classification/pictograms: Not specified for this item; refer to SDS.
General laboratory safety guidance (not item-specific; follow your institutional EHS and the SDS):
PPE: lab coat, safety glasses, and suitable chemical-resistant gloves (e.g., nitrile). Work in a chemical fume hood when handling powders/solutions or during weighing and transfers.
Avoid inhalation of dust/aerosols and skin/eye contact. Wash thoroughly after handling. Do not eat, drink, or smoke in work area.
Storage incompatibilities: Until the structure and functional groups are confirmed via CoA/SDS, segregate from strong oxidizers and strong acids/bases as a precaution. Keep container tightly closed.
First aid (overview): In case of eye/skin contact, rinse with water for ≥15 min; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical advice. Always consult the SDS for authoritative instructions.
Waste: Collect as organic chemical waste. Do not discharge to drains. Label containers with full product identifier and hazards per SDS.
Note: This product is for research use only.
Solvent Selection
Item-specific solubility data are not provided; refer to the CoA/Spec Sheet. The following is general best practice for small-molecule library compounds:
Primary stock solvent: DMSO is typically preferred for bioactive small molecules due to broad solvency, low volatility, and biological compatibility at ≤1% v/v.
Secondary options: DMF, acetonitrile, ethanol, or methanol can be used if DMSO is incompatible with the assay. Verify stability (no rapid hydrolysis/oxidation) by LC.
Aqueous use: For buffers (e.g., PBS, HEPES), first dissolve in DMSO at high concentration (e.g., 10–50 mM) then dilute into buffer to the working concentration, keeping final DMSO ≤0.1–1% as your biology allows.
Surfactants: For highly hydrophobic entities, 0.01–0.1% nonionic surfactant (e.g., Tween 20 or Pluronic F-68) in the assay buffer can mitigate precipitation; confirm no assay interference.
Filtration: If particulate is observed, sterile-filter through a 0.22 μm PTFE or PES membrane compatible with the solvent system.
Light/air sensitivity: Unknown for this item; minimize exposure during solution prep as a precaution and discard solutions showing discoloration/precipitation.
Quick comparison (general):
DMSO: highest solvency, biologically tolerated at low %, hygroscopic.
EtOH/MeOH: convenient, but limited for very lipophilic molecules and can perturb some assays.
Storage & Reconstitution
Storage conditions (provided): Room temperature.
Shipped in: Not specified for this item; refer to CoA/Spec Sheet.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
General guidance (not item-specific; defer to CoA/SDS if different):
Keep container tightly closed in a dry place. Protect from excessive humidity and direct light until more is known about photostability.
Upon first opening, consider subdividing into single-use aliquots under dry conditions (e.g., glove bag or desiccator) to minimize moisture exposure.
Reconstitution: Prepare concentrated stock solutions in anhydrous DMSO (typical 10–50 mM). Mix thoroughly; if needed, warm gently (≤37 °C) and/or brief sonication to aid dissolution. Do not overheat.
Storage of solutions: Store DMSO stocks in sealed, inert-atmosphere vials when possible. For most discovery compounds, short-term storage at room temperature or 4 °C is acceptable; for longer-term stability, −20 °C is commonly used. Avoid repeated freeze–thaw by aliquoting.
Inspection: Discard solutions showing precipitation, discoloration, or new LC/MS peaks relative to the neat material.
Note: For research use only. Always prioritize lot-specific instructions on the CoA and SDS for A607328 (ABL127).
Structure & Identity
Item: ABL127 (SKU: A607328)
Category: Small molecule/compound library member (Moligand grade)
Item-specific identifiers (as provided):
CAS: A607328 (catalog placeholder; not a registry CAS)
PubChem CID: 24856225 (as provided)
InChIKey: 464506 (as provided)
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Structural features:
Functional groups, ring systems, stereochemistry, and atom connectivity cannot be detailed because no structural string (SMILES/InChI) or formula is provided for this catalog entry. See the product’s CoA/Spec Sheet for definitive structure confirmation and quality release data.
Practical identity guidance:
Cross-reference the provided PubChem CID in your informatics workflow if structural confirmation is needed prior to procurement. Always reconcile any third‑party structure with the Certificate of Analysis (the CoA governs).
Synthetic Utility
Synthetic reagent functionality is not defined for ABL127 in the Product Data. Without a disclosed structure or functional group set, we cannot advise on its behavior in named synthetic transformations or its value as a building block.
General guidance (for researchers considering derivatization in SAR studies):
If structural information is obtained from the CoA or literature linked to the provided PubChem CID, map vectors for diversification and identify potential liabilities (hydrolysis-prone motifs, redox-sensitive moieties).
Confirm stability in common reaction media before attempting late-stage diversification (e.g., compatibility with bases, acids, transition-metal catalysts).
For analytical tracking during modifications, develop orthogonal methods (UPLC-UV, LC-MS, possibly 1H/13C NMR) and reference the original lot as a benchmark.
Absent item-specific details, treat ABL127 primarily as a finished tool compound rather than a general synthetic intermediate.
Target Specificity
No target, selectivity profile, or binding constant data are provided for ABL127 in the Product Data. Therefore, no claims about protein targets, pathways, or species selectivity can be made for this item.
Guidance (general, not item-specific):
If a putative target is identified from literature via the provided PubChem CID, validate under your conditions using orthogonal assays (biochemical potency, CETSA/DSF, pull‑down/ABPP, or genetic knockdown/knockout controls).
Assess off-targets with panel screens or carefully chosen counterscreens to distinguish true target engagement from assay artifacts.
Please consult the CoA/Spec Sheet and SDS for authoritative information.
Frequently Asked Questions
What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at room temperature.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is A607328. InChIKey ZRHWCAFAIHTQKD-KRWDZBQOSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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