GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Rabbit anti Human ALEX1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Specifications
Product Name
ALEX1 Antibody - Primary antibody, specific to ARMCX1, Rabbit IgG
Synonyms
ARMCX 1 AD032 | ALEX1 | ARM protein lost in epithelial cancers on chromosome X 1 | Arm protein lost in epithelial cancers X chromosome 1 | Armadillo repeat containing X linked 1 | Armadillo repeat containing X linked protein 1 | Armadillo repeat-containin
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
ARMCX1
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ARMCX1
Positive Control
WB: Rat kidney lysates. IHC: Human brain formalin tissue. IF/ICC: HeLa cells
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ALEX1 Antibody (Ab088136) - Western Blot All lanes: ALEX1 Antibody (Ab088136) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 46 kDa Exposure time: 60.0 s
ALEX1 Antibody (Ab088136) - IHC Immunohistochemical analysis of ARMCX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALEX1 Antibody (Ab088136) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ALEX1 Antibody (Ab088136) - IF/ICC Immunofluorescent analysis of ARMCX1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALEX1 Antibody (Ab088136) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Application
Application
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
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Reconstitution Calculator
Reviews
Customer Reviews
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