Immobilized Cephalexin Acylase, CAS No.9014-06-6

CAS: 9014-06-6 Cat. No.: A1435573 EC Number: 232-753-8

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, >50 U/g wet weight

Bioactivity

>200 U/g wet weight

Overview

 It produces cephalexin from 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and PGM (phenylglycine methyl ester).

Standard

Activity: >200 U/g wet weight

Size of immobilization support: 250~500μm (>95%)

Water content <65%

Enzyme assay: One unit (U) represents the amount of enzyme required for producing 1μmol cephalexin per min in 50mM K-phosphate buffer containing 40mM 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and 40mM PGM (phenylglycine methyl ester), pH 7.0 at 28°C.

Application 

Cephalexin production from 7-ADCA and PGM.

Characteristics 

· High production of cephalexin

· Low degradation activity of cephalexin and PGM

· Fast enzyme reaction (reaction completed in 90 minutes)

· High stability (reusable more than 500 cycles)

· High production rate of cephalexin (more than 98.5%)

Scheme for enzyme reaction 

Optional reaction conditions (Temperature, pH) 

Production instruction

Category	Contents
Materials	·Synthesizing enzyme: Immobilized Cephalexin Acylase (200U/g wet, 61% water content) ·7-ADCA , PGM·HCl ·Water ·Ammonia hydroxide(28%)
Condition	·pH: 7.5-8.0 ·Temperature: 10°C ·7-ADCA : PGM: 350mM : 392mM(1:1.10) ·Enzyme: 1.0 fold of 7-ADCA ·Reaction solution: Water
Step	1. Remove the water after washing immobilized enzyme with water more than five times. 2. Dilute 7-ADCA(350mM) and PGM·HCl (392mM) with 90ml water and adjust pH to 7.5 withammonium hydroxide. 3. Pour 6.75g wet enzyme in conversion reactor. 4. Proceed synthetic reaction at 10°C and 550rpm after pouring substrates. 5. Extract every 10 minutes after 1 hour. Stop the reaction if conversion rate of 7-ADCA is more than 98.5%. 6. Take the reaction solution in reactor.

Fig 3. Cefradine production by Immobilized Cephalexin Acylase

Specifications

Product Name

Immobilized Cephalexin Acylase, CAS No.9014-06-6

Synonyms

Cephalexin acylase (Immobilized)

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, >50 U/g wet weight

Bioactivity

>200 U/g wet weight

CAS

9014-06-6

Enzyme Commission Number

EC 3.5.1.11

Molecule Type

Enzyme

Storage and Shipping

Concentration

>50 U/g wet weight

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Unit definition

One unit (U) represents the amount of enzyme required for producing 1μmol cephalexin per min in 50mM K-phosphate buffer containing 40mM 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and 40mM PGM (phenylglycine methyl ester), pH 7.0 at 28°C.