AS2863619 - ≥98% , CAS No.2241300-51-4

CAS: 2241300-51-4 Cat. No.: A414258 Formula: C₁₆H₁₄Cl₂N₈O Molecular Weight: 405.24 PubChem CID: 139600293

GRADE & PURITY ≥98%

Synonyms

1,​2,​5-​Oxadiazol-​3-​amine,4-​[1-​(2-​methyl-​1H-​benzimidazol-​6-​yl)​-​1H-​imidazo[4,​5-​c]​pyridin-​2-​yl]​-​,hydrochloride (1:2)

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

Information

AS2863619 AS2863619 is a small-molecule cyclin-dependent kinase CDK8/19 inhibitor with IC50 of 0.6099 nM and 4.277 nM, respectively. AS2863619 is a potent Foxp3 inducer in T conv cells.

Targets

CDK8 (Cell-free assay); CDK19 (Cell-free assay) 0.6099 nM; 4.277 nM

In vitro

AS2863619 induction of Foxp3 in vitro does not require exogenous TGF-β. AS2863619-induced iTreg cells can be induced in the presence of inflammatory cytokines such as IL-4, IL-6, and IFN-γ, which appear to hamper Foxp3 gene activation via activation of STATs. AS2863619 is able to generate iTreg cells not only from naïve Tconv cells but also from effector/memory Tconv cells. Furthermore, AS2863619 cannot induce Foxp3 in CDK8 and CDK19 double-deficient T cells. AS2863619-induced iTreg cells require TCR and IL-2 stimulation for their generation and are devoid of Treg-type DNA hypomethylation, which is present in nTreg cells and required for stable Treg function.

In vivo

AS2863619 induces in vivo Foxp3 induction in antigen-activated T cells. In vivo AS2863619-induced Foxp3+ T cells are able to gradually acquire Treg-specific epigenetic alterations, differentiating into functionally stable Foxp3+ pTreg cells having a more nTreg-like gene expression profile.

Cell Research(from reference)

Cell lines：PBMCs, Human CD4+ T cells, lymph node cells derived from eFox mice 

Concentrations：1.0 μM 

Incubation Time：24 h, 0-44 h 

Specifications

Synonyms

1,​2,​5-​Oxadiazol-​3-​amine,4-​[1-​(2-​methyl-​1H-​benzimidazol-​6-​yl)​-​1H-​imidazo[4,​5-​c]​pyridin-​2-​yl]​-​,hydrochloride (1:2)

Specifications & Purity

≥98%

Biochemical and Physiological Mechanisms

AS2863619 is a small-molecule cyclin-dependent kinase CDK8/19 inhibitor with IC50 of 0.6099 nM and 4.277 nM, respectively. AS2863619 is a potent Foxp3 inducer in T_conv cells.

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Purity

≥98%

Product Properties

ALogP

2.468

HBD Count

2

Rotatable Bond

2

Names and Identifiers

Pubchem Sid

504773569

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/504773569

Canonical Smiles

CC1=NC2=C(N1)C=C(C=C2)N3C4=C(C=NC=C4)N=C3C5=NON=C5N.Cl.Cl

IUPAC Name

4-[1-(2-methyl-3H-benzimidazol-5-yl)imidazo[4,5-c]pyridin-2-yl]-1,2,5-oxadiazol-3-amine;dihydrochloride

InChIKey

LHUAALWRORLTCJ-UHFFFAOYSA-N

INCHI

1S/C16H12N8O.2ClH/c1-8-19-10-3-2-9(6-11(10)20-8)24-13-4-5-18-7-12(13)21-16(24)14-15(17)23-25-22-14;;/h2-7H,1H3,(H2,17,23)(H,19,20);2*1H

PubChem CID

139600293

Molecular Weight

405.24

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro Water: 81 mg/mL (199.88 mM); DMSO: 60 mg/mL (148.06 mM); Ethanol: 4 mg/mL (9.87 mM)
• Sensitivity: Moisture sensitive
• DMSO(mg / mL) Max Solubility: 60
• DMSO(mM) Max Solubility: 148.060408646728
• Water(mg / mL) Max Solubility: 81
• Water(mM) Max Solubility: 199.881551673083
• Molecular Weight: 405.200 g/mol
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 2
• Exact Mass: 404.067 Da
• Monoisotopic Mass: 404.067 Da
• Topological Polar Surface Area: 124.000 Ų
• Heavy Atom Count: 27
• Formal Charge: 0
• Complexity: 495.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

XLogP3