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Beta-1,4-Galactosyltransferase 1 (Y285L)

Cat. No.: B1438840
COA
Beta-1,4-Galactosyltransferase 1 (Y285L)
AVAILABLE TO ORDER
GRADE & PURITY Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. His Tag ? His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. expressed in HEK293；>1000 U/mg protein；Protein concentration: See COA
Accession #
P08037
Protein Tag
C-His
Expression system
HEK293
Bioactivity
>1000 U/mg protein
★
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
B1438840-10μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$159.90
50μg
B1438840-50μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$489.90
100μg
B1438840-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$869.90
1mg
B1438840-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$4,299.90
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SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Overview Specifications Storage and Shipping Documentation Advanced Data Certificates（CoA,COO,BSE/TSE and Analysis Chart) Documents & Articles Solution Calculators Reviews
🧪

Why this grade

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,His-Tag,expressed in HEK293；>1000 U/mg protein；Protein concentration: See COA ActiBioPure™,Bioactive,High Performance,His Tag,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Beta-1,4-galactosyltransferase 1 (Y285L) is a β1,4-N-acetylgalactosaminyltransferase that can efficiently transfer GalNAc or its derivatives such as GalNAz to terminal GlcNAc residues. The latter can be applied to antibody-drug conjugates (ADCs) for targeted cancer therapy and other applications.


Reaction Formula 


Product Properties

Source:  HEK293

Label: C-terminal His Tagged

Molecular weight: 50.8 kDa

Storage Buffer: 20 mM Tris-HCl, 200 mM NaCl, 20% Glycerin, pH 7.4

Enzyme activity: >1000 U/mg protein

Unit Definition:  The amount of enzyme (mg) required to transfer 1 nmol of GalNAz to GlcNAc per minute at 37℃.


Transport and Storage Methods

Transported on ice packs or dry ice; stored at -20℃; valid for 12 months.

Recommended Usage (can be adjusted according to actual experimental conditions)

1. Reaction System: 50 mM Tris-HCl (pH 7.5), 6 mM MnCl₂

2. Reaction Reagents: ① UDP-GalNAz:antibody (molar ratio) = 300:1; ② Enzyme addition amount: enzyme/antibody = 5%

3. Prepare the reaction solution according to the above conditions, incubate at 37℃ for 48 hours; then place at -15 to -25℃ to terminate the reaction and conduct sample detection.


Precautions Recommended Usage

Please pay attention to experimental safety and operate with gloves. This product is for research use only!

Specifications

Product Name
Beta-1,4-Galactosyltransferase 1 (Y285L)
Synonyms
B4GALT1 | GT1 | GTB | CDG2D | GGTB2 | CLDLFIB | B4GAL-T1 | beta4Gal-T1
Grade
ActiBioPure™, Bioactive, High Performance, His Tag, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, His-Tag, expressed in HEK293；>1000 U/mg protein；Protein concentration: See COA
Biochemical and Physiological Mechanisms
Beta-1, 4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carb
Bioactivity
>1000 U/mg protein
Accession #
P08037
Predicted molecular weight
50.8 kDa
Enzyme Commission Number
EC 2.4.1-
Molecule Type
Enzyme
Storage and Shipping
Concentration
expressed in HEK293；>1000 U/mg protein；Protein concentration: See COA
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Unit definition
The amount of enzyme (mg) required to transfer 1 nmol of GalNAz to GlcNAc per minute at 37℃.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
His Tag Grade Reagents
Categories: Specifications, Grading and Purity
Tags:
His Tag
Industrial Biocatalysis Reshaping Manufacturing: Application Systems and Technological Evolution in Industrial Enzymology
Categories: Technical articles
Tags:
Biocatalysis industrial enzymology enzyme engineering immobilized enzymes cofactor regeneration continuous manufacturing green chemistry chiral synthesis multi-enzyme cascades biomanufacturing
Molecular Mechanisms of Glycosylation-Mediated Regulation of Cell Surface Signaling
Categories: Technical articles
Tags:
Glycosylation Signal Transduction Lectins Glycan Modification Cell Surface Signaling N-Glycosylation O-Glycosylation Glycolipids Glycocalyx Receptor Clustering
Regulatory Mechanisms of Extracellular Matrix Glycosylation in Tissue Homeostasis Maintenance and Remodeling
Categories: Technical articles
Tags:
Glycosylation Hyaluronic acid Glycosaminoglycans Extracellular matrix Proteoglycans Heparan Sulfate Tissue Homeostasis Matrix Remodeling Growth Factor Storage Inflammatory Regulation
Cell Surface Glycan Modification Research: Glycosyltransferases, Glycosidases, and Glycocalyx Functional Analysis
Categories: Technical articles
Tags:
Glycosidase Sialylation Glycomics Glycocalyx Fucosylation Cell surface glycan modification Glycosyltransferase Lectin probe
Solution Calculators
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