Colforsin (Forskolin, HL 362) - Moligand™, 10mM in DMSO , CAS No.66575-29-9

CAS: 66575-29-9 Cat. No.: C409070 Molecular Weight: 410.5 Beilstein Registry Number: 1692720 EC Number: 266-410-9

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

Coleonol, Colforsin | 1H-Naphtho[2,1-b]pyran-1-one, 5-(acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-, (3R,4aR,5S,6S,6aS,10S,10aR,10bS)-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Colforsin (Forskolin, HL 362) Colforsin (Forskolin, HL 362, Coleonol, Colforsin) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physi
In vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (G_s)–AC complexes. Activation of _s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of _s-Forskolin potentiation of AC activity. Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin\'s potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin\'s direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma.

In vivo

Cell Data

cell lines：Human gastric cancer cells NCI-N87

Concentrations：

Incubation Time：

Powder Purity:≥97%

Specifications

Synonyms

Coleonol, Colforsin | 1H-Naphtho[2, 1-b]pyran-1-one, 5-(acetyloxy)-3-ethenyldodecahydro-6, 10, 10b-trihydroxy-3, 4a, 7, 7, 10a-pentamethyl-, (3R, 4aR, 5S, 6S, 6aS, 10S, 10aR, 10bS)-

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Colforsin (Forskolin, HL 362, Coleonol, Colforsin) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Names and Identifiers

• Isomeric SMILES: CC(=O)O[C@H]1[C@H]([C@@H]2[C@]([C@H](CCC2(C)C)O)([C@@]3([C@@]1(O[C@@](CC3=O)(C)C=C)C)O)C)O
• WGK Germany: 3
• RTECS: QL6150000
• Molecular Weight: 410.5
• Beilstein: 1692720
• Reaxy-Rn: 8940142
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8940142&ln=

Associated Targets(Human)

RXFP1 Tchem Relaxin receptor 1 (1 Activities)

Discover Target: RXFP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ADCY8 Tchem Adenylate cyclase type 8 (1 Activities)

Discover Target: ADCY8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ADCY1 Tchem Adenylate cyclase type 1 (2 Activities)

Discover Target: ADCY1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro
• Specific Rotation[α]: -17°
• Melt Point(°C): 224.0 to 228.0°C

References

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2. Fangwei Yang, Yixuan Li, Yunfei Xie, Weirong Yao, Fazheng Ren.  (2022)  Diethyl phosphate disrupts hypothalamus-pituitary-adrenal axis endocrine hormones via nuclear receptors GR and Nur77: Integration of evidences from in vivo, in vitro and in silico approaches.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:35777568] [10.1016/j.scitotenv.2022.157015]

3. Ye-ying Jiang, Rong-yun Wei, Kai Tang, Zhen Wang, Ning-hua Tan.  (2022)  Ginsenoside Rg1 promotes neurite growth of retinal ganglion cells through cAMP/PKA/CREB pathways.  Journal of Ginseng Research,      [PMID:38465221] [10.1016/j.jgr.2022.05.002]

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6. Jinping Wang, Jianmin Wu, Zishen Lin, Ning Ma, Zhaoyue Men, Chao Zhang, Xi Ma, Hongyu Zheng.  (2024)  Dietary sodium butyrate and forskolin promote cell proliferation to resist Citrobacter rodentium infection by lysozyme upregulation.  Journal of Functional Foods,      [PMID:] [10.1016/j.jff.2023.105993]

7. Jing Xia, Xiaoyan Li, Hongyu Zhu, Xiaohui Zhou, Ji Chen, Qihong Li, Shupeng Li, Haichen Chu, Mingxin Dong.  (2024)  The μ-opioid receptor-mediated Gi/o protein and β-arrestin2 signaling pathways both contribute to morphine-induced side effects.  EUROPEAN JOURNAL OF PHARMACOLOGY,      [PMID:38278466] [10.1016/j.ejphar.2024.176333]

8. Shiyan Liu, Xuefeng Zhang, Xin Gong, Jinxin Yu, Tao Lin, Qian Xiang, Xinnian Zeng, Jiali Liu.  (2025)  molecular and pharmacological characterization of the dopamine receptors in the oriental fruit fly, Bactrocera dorsalis.  INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,      [PMID:40245998] [10.1016/j.ibmb.2025.104312]

9. Haopeng Lin, Xin Liu, Feng Zhang, Deqi Chen, Xiaoxiao Xie, Qifei Dong, Jiawei Yan, Jiaxiang Yin, Zirong Bi, Zhihua Dou, Yingling Jiang, Kuo Jiang, Tongran Zhang, Peng Xue, Wei Peng, Lihua Chen, Tao Xu, Zonghong Li, Yanying Guo, Huisheng Liu.  (2025)  Restoration of PKM1 improves functional maturation of human stem-cell derived-β cell by regulating PEP metabolism.  Nature Communications,      [PMID:41423449] [10.1038/s41467-025-67635-2]

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