Concanavalin A, AF594 conjugate, CAS No.11028-71-0

CAS: 11028-71-0 Cat. No.: np231274 EC Number: 234-258-2

GRADE & PURITY BioReagent, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Overview

Concanavalin A (ConA) lectin is isolated from Jack beans (Canavalia ensiformis) and purified by affinity chromatography. The molecular weight of the lectin is 104 kDa. The lectin has broad applicability and is the most widely used lectin within molecular biology research. ConA binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids.

We offer a broad selection of concanavalin A conjugates for the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose. Fluorescent Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. All conjugates are supplied essentially free of unconjugated fluorochromes.

Guideline for Use

1.     Training protocols vary with application. The appropriate dilution of concanavalin A conjugate should be determined empirically. A final concentration of 5‑200 µg/mL is typically satisfactory for immunohistochemical applications.

2.     Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Specifications

Product Name

Concanavalin A, AF594 conjugate, CAS No.11028-71-0

Synonyms

Concanavalin A from Canavalia ensiformis (Jack bean), AF594 conjugate | Concanavalin A (Con A), AF594 | Lectin from Concanavalin A (Canavalia ensiformis), AF594 Labeled | AF594 conjugate of concanavalin A | Concanavalin A, AF594 conjugate | Concanavalin A

Grade

BioReagent, Suitable for Immunofluorescence(IF)

Specifications & Purity

Suitable for Immunofluorescence(IF), BioReagent, 1.0 mg/mL

Conjugation

AF594

Excitation (nm)

Yellow-Green Laser(594nm)

Emission(nm)

618nm

Ex/Em(nm)

CAS

11028-71-0

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Concanavalin A, AF594 conjugate (np231274)

Concanavalin A, AF594 conjugate (np231274) - IF/ICC 

HeLa cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Concanavalin A, AF594 conjugate (np231274) (red) at 1/200 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.  

Application

• Application: Dilution info
• IF/ICC:
  1/10-1/200

References

1. Mengling Zhong, Mingmei Feng, Xinlin Chen, Yuxin Zhang, Yupeng Wang, Peizhi Yuan, Tianyao Zhang, Zhipeng Han, Chenglun Tang, Qi Zhang, Yang Sun.  (2025)  Cordycepin attenuated cyclophosphamide (CTX)-induced immunosuppression in mice via EGFR/Nrf2 antioxidant signaling pathway.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:40695148] [10.1016/j.intimp.2025.115235]

2. Yifeng Tan, Shaoxiong Zhang, Yangyang Cheng, Shiyong Zhang.  (2025)  Cross-linked selenolipoic acid nanoplatform modulates intracellular and extracellular reactive oxygen species for cancer immunotherapy.  JOURNAL OF CONTROLLED RELEASE,      [PMID:40818722] [10.1016/j.jconrel.2025.114130]

3. Mintong Tian, Yuman Guo, Meng Zhou, Purui Yu, Mingming Li, Zehua Li, Qipeng Yuan.  (2025)  Efficient Preparation of High-Purity Sulforaphene: Selective Immobilization of Myrosinase by Designing a Concanavalin A-Loaded Metal–Organic Framework.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:40855066] [10.1021/acs.jafc.5c06629]

4. Ya Li, Yao Lu, Yang Chen, Pei Zhang, Nengqin Jia.  (2023)  Fullerene-promoted organic-inorganic hybrid electrochemiluminescence biosensor for sensitive detection of concanavalin A.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2023.134311]

5. Qin Yang, Zhenkun Zhang, Lin Wang, Xiwen Xing, Jiahai Zhou, Long Li.  (2023)  Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening.  Nanomaterials,  13  (14): (2064).  [PMID:37513075] [10.3390/nano13142064]

6. Tongtong Zhang, Shuai Xu, Xingyu Lin, Jiyang Liu, Kai Wang.  (2023)  Label-Free Electrochemical Aptasensor Based on the Vertically-Aligned Mesoporous Silica Films for Determination of Aflatoxin B1.  Biosensors-Basel,  13  (6): (661).  [PMID:37367026] [10.3390/bios13060661]

7. Jinxia ZHU, Hongxiu CHEN, Jianjiao CUI, Xiaorui ZHANG, Guangwei LIU.  (2023)  Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation.  EXPERIMENTAL ANIMALS,      [PMID:36927981] [10.1538/expanim.22-0171]

8. Zhengge Wang, Chengyu Fu, Yingtao Gao, Zhaoji Wu, Weiqing Chen, Bowen Hu, Siqi Xu, Zhigang Zhang, Peng Yang.  (2023)  Dual functional antifouling and bactericidal proteinaceous coating.  COLLOIDS AND SURFACES B-BIOINTERFACES,      [PMID:36889106] [10.1016/j.colsurfb.2023.113239]

9. Yufang Li, Shu Wang, Haojie Ding, Kang Xiao, Xia Huang.  (2023)  Quorum sensing-Fe metabolism interplay affects biofouling on reverse osmosis membrane: Evidences from microbial shift and structure alteration.  DESALINATION,      [PMID:] [10.1016/j.desal.2023.116416]

10. Haoran Jiang, Xianxin Wen, Xue Zhang, Bingyu Zhang.  (2022)  Concanavalin A inhibits human liver cancer cell migration by regulating F‑actin redistribution and assembly via MAPK signaling pathway.  Oncology Letters,  24  (5): (1-12).  [PMID:36276493] [10.3892/ol.2022.13525]

11. Zhaoquan Zheng, Bingyu Wang, Jing Chen, Yan Wang, Ziyue Miao, Cenyao Shang, Qiang Zhang.  (2021)  Facile synthesis of Antibacterial, Biocompatible, quaternized Poly(ionic liquid)s with pendant saccharides.  EUROPEAN POLYMER JOURNAL,      [PMID:] [10.1016/j.eurpolymj.2021.110702]

12. Die Li, Jing Chen, Mei Hong, Yan Wang, David M. Haddleton, Guang-Zhao Li, Qiang Zhang.  (2021)  Cationic Glycopolymers with Aggregation-Induced Emission for the Killing, Imaging, and Detection of Bacteria.  BIOMACROMOLECULES,      [PMID:33909978] [10.1021/acs.biomac.1c00298]

13. Xiu-yan Li, Pin Peng, Wei-kang Wang, Si-yuan Wang, Lei Feng, Yan-chen Zhang, Juan Xu.  (2021)  Particle electrode materials dependent tetrabromobisphenol A degradation in three-dimensional biofilm electrode reactors.  ENVIRONMENTAL RESEARCH,      [PMID:33811867] [10.1016/j.envres.2021.111089]

14. Xia Wang, Jianbo Zhu, Baida Kong, Bingnan He, Lai Wei, Yuanxiang Jin, Yudong Shan, Weitao Wang, Chunqiang Pan, Zhengwei Fu.  (2019)  C9–13 chlorinated paraffins cause immunomodulatory effects in adult C57BL/6 mice.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:31026635] [10.1016/j.scitotenv.2019.04.199]

15. Pang Juan, Gao Ziyu, Tan Huaping, Mao Xincheng, Wang Huiming, Hu Xiaohong.  (2019)  Design, Synthesis, Investigation, and Application of a Macromolecule Photoswitch.  Frontiers in Chemistry,      [PMID:30873401] [10.3389/fchem.2019.00086]

16. Jianwen Hou, Runhai Chen, Jingchuan Liu, Haozheng Wang, Qiang Shi, Zhirong Xin, Shing-Chung Wong, Jinghua Yin.  (2018)  Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern.  Journal of Materials Chemistry B,  6  (29): (4792-4798).  [PMID:32254306] [10.1039/C8TB01441H]

17. Chaohong Gao, Guo Lin, Zhixian Lei, Qiong Zheng, Jiashi Lin, Zian Lin.  (2017)  Facile synthesis of core–shell structured magnetic covalent organic framework composite nanospheres for selective enrichment of peptides with simultaneous exclusion of proteins.  Journal of Materials Chemistry B,  5  (36): (7496-7503).  [PMID:32264225] [10.1039/C7TB01807J]

18. Xiaojian Li, Yaoguang Wang, Li Shi, Hongmin Ma, Yong Zhang, Bin Du, Dan Wu, Qin Wei.  (2017)  A novel ECL biosensor for the detection of concanavalin A based on glucose functionalized NiCo2S4 nanoparticles-grown on carboxylic graphene as quenching probe.  BIOSENSORS & BIOELECTRONICS,      [PMID:28475956] [10.1016/j.bios.2017.04.050]

19. Lu Mengdi, Liang Yuzhang, Qian Siyu, Li Lixia, Jing Zhenguo, Masson Jean-Francois, Peng Wei.  (2016)  Optimization of Surface Plasmon Resonance Biosensor with Ag/Au Multilayer Structure and Fiber-Optic Miniaturization.  Plasmonics,  12  (3): (663-673).  [PMID:] [10.1007/s11468-016-0312-4]

20. Shenguang Ge, Lina Zhang, Yan Zhang, Haiyun Liu, Jiadong Huang, Mei Yan, Jinghua Yu.  (2015)  Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing.  TALANTA,      [PMID:26459438] [10.1016/j.talanta.2015.05.008]

21. Jing Liu, Yan Ping Chen, Penglin He, Ziyu Ding, Yun Guo, Songhe Cui, Chao Ma, Zhiping Xie, Sun Xia, Yin Zhang, Ye Liu, Yuan Liu.  (2024)  A novel olfactory biosensor based on ZIF-8@SWCNT integrated with nanosome-AuNPs/Prussian blue for sensitive detection of hexanal.  FOOD CHEMISTRY,      [PMID:38266411] [10.1016/j.foodchem.2023.138349]

22. Mengyuan Chen, Chong Wang, Jing Tan, Jin Wang, Chen Wang.  (2024)  Cell membrane-inspired COF/AAO hybrid nanofluidic membrane with ion current rectification properties for ultrasensitive detection of E. coli.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136719]

23. Chenze Lu, Gongyan Wang, Xiaoxiao Zhang, Zhihui Fang, Xinyuan Wang, Fan Tang, Kang Ning, Min Xu, Jing Wang, Han Jiang, Ran Tao, Pei Xu.  (2025)  Cost-effective, label-free electrochemical aptasensors for rapid detection of concanavalin A with screen printed electrodes.  FOOD CHEMISTRY,      [PMID:39977999] [10.1016/j.foodchem.2025.143338]

24. He Li, Changxin Zheng, Yanru Zheng, Kai Wen, Yingjiu Zhang.  (2024)  Distinct functional diversity of branched oligosaccharides as chaperones and inhibitory-binding partners of amyloid beta-protein and its aggregates.  NEUROPHARMACOLOGY,      [PMID:39251087] [10.1016/j.neuropharm.2024.110141]

25. Jinming Zhang, Jihang Zhang, Jiale Chen, Xiao Zhang, Jinglan Wu, Pengpeng Yang, Fengxia Zou, Hanjie Ying, Wei Zhuang.  (2025)  Exploiting unique NP1 interface: Oriented immobilization via electrostatic and affinity interactions in a tailored PDA/PEI microenvironment enhanced by concanavalin A.  TALANTA,      [PMID:39798416] [10.1016/j.talanta.2025.127528]

26. Chendi Gao, Ensong Zhang, Xinyu Bian, Qiaoran Li, Chunming Wang, Guang Yang, Ming Jiang, Guosong Chen.  (2024)  One-Pot Fabrication of Supramolecular Synthetic Protein Hydrogel with Tissue-like Integrated Dynamic Features.  BIOMACROMOLECULES,      [PMID:38386431] [10.1021/acs.biomac.3c01451]

27. Jin-Xian Fu, Jiao Jiao, Qing-Yan Gai, Yu-Jie Fu, Mo-Nan Wen, Xiao-Qing Wang, Jing He.  (2025)  Sporopollenin-chitosan microspheres loaded with an endophytic fungus Talaromyces neorugulosus R-209 for promoting development and controlling root rot in pigeon pea.  JOURNAL OF PLANT PHYSIOLOGY,      [PMID:40700996] [10.1016/j.jplph.2025.154569]

28. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]

29. Nan Chen, Shuai Zhao, Zhiyong Yin, Samiullah Khan, Jianjun Guo, Min Zhao.  (2026)  The potential of Protaetia brevitarsis as a functional food that enhances immune function and gut microbiota in mice.  iScience,      [PMID:41858617] [10.1016/j.isci.2026.114939]

30. Xiaochu Chen, Minxing Huang, Jianbin Chen, Xiaopeng Wang, Qiaoyun Cheng, Zhaohua Ping, Guihua Wang, Yufeng Gao.  (2026)  Site-specific immobilization of tyrosinase on magnetic polystyrene nanospheres as dual-functional biosensor for enhanced colorimetric analysis of atrazine in vegetables.  TALANTA,      [PMID:41690017] [10.1016/j.talanta.2026.129522]

31. Jing Sun, Hong Shu, Xiaoyan Wu, Tingting Liu, Guangyue Li, Zhao Cui, Ting Li, Xiaowei Zhou, Zhongran Dai, Qian Li.  (2026)  Quorum sensing-driven biofilm cathode of Shewanella oneidensis for enhanced uranium adsorption: Elucidating the role of exogenous AHLs and validation in actual uranium-contaminated groundwater.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:41702164] [10.1016/j.jhazmat.2026.141485]