DAPI Staining Solution - BioReagent, for microscopy, sterile-filtered, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Cat. No.: D1372407

GRADE & PURITY Sterile-filtered, BioReagent, for Microscopy, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Synonyms

DAPI | DAPI Staining Solution | DAPI Reagent | DAPI Stain | DAPI Solution

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry, IF/ICC

Overview

  The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding of DAPI to dsDNA produces a ~20-fold fluorescence enhancement, apparently due to the displacement of water molecules from both DAPI and the minor groove. DAPI also binds RNA, however in a different binding mode. The DAPI/RNA complex exhibits a longer-wavelength fluorescence emission maximum than the DAPI/dsDNA complex (~500 nm versus ~460 nm) and a quantum yield that is only about 20% as high.

Attention:

1. DAPI is a known mutagen and should be handled with care. The dye must be disposed of safely and in accordance with applicable local regulations.

2. The fluorescent dye has the problem of quenching, and it is recommended to finish the analysis on the same day after dyeing.

Instructions for Use：

1. It is recommended to titrate the DAPI Staining Solution to obtain optimal results as applications vary. 

2. Dilute the D1372407 with FACs buffer to the appropriate working solution (0.1-10 μg/mL).

3. Staining of Live Cells for Viability Analysis:

a. Obtain a single cell suspension, add 100 μL working solution to a 96-well and 1 mL to a 6-well;

b. Incubate 5 minutes at room temperature;

c. Proceed to analysis by flow cytometry or fluorescence analysis.

4. Staining of Fixed Cells for DNA Content Analysis:

a. Wash cells once to remove the fixation. DAPI staining is normally performed after all other staining;

b. Add appropriate volume of DAPI working solution (100 μL for a 96-well and 1 mL for a 6-well);

c. Incubate 5 minutes at room temperature;

d. Proceed to analysis by flow cytometry or fluorescence analysis.

Specifications

Synonyms

DAPI | DAPI Staining Solution | DAPI Reagent | DAPI Stain | DAPI Solution

Specifications & Purity

BioReagent, for microscopy, sterile-filtered, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Stability And Storage

Store at -20°C long term (12 months). Store in the dark.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Microscopy, Sterile-filtered, Suitable for Immunofluorescence(IF)

Images

Flow Cytometry: DAPI Staining Solution (D1372407)

DAPI Staining Solution (D1372407) - Flow Cytometry
Dot plot showing the viability of bovine CD8-overexpressed HEK293 cells stained with D1372407 (Right). Left - Unstained cells.
1x106 HEK293 cells transiently transfected with bovine CD8 for 2-days were harvested, stained with 1.0 μg/mL DAPI Staining Solution (D1372407) in 100 μL FACS buffer for 10 min at 4℃ in the dark and analyzed by flow cytometry. Cell debris were excluded from the analysis based on scatter signals. Dead cells are positive for DAPI and thus can be excluded from analysis.

Chemical and Physical Properties

• Sensitivity: Light-sensitive