Elastase, CAS No.39445-21-1

CAS: 39445-21-1 Cat. No.: np001097 EC Number: 254-453-6 PubChem CID: 363902459

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

Accession #

P00772

Bioactivity

≥50 U/mg protein

Overview

Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.

Biochemical Physiological 

Actions Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Form: 2X crystallized. Aqueous suspension containing 0.01% NaN₃ as preservative. 

Greater that 50 units per mg of protein on the substrate elastin-porcein. One unit will solubilize 1 mg of elastin in 20 minutes at pH 8.8 and 37°C.

Greater that 4 units per mg of protein on the substrate Suc-(Al)₃-pNA. One will hydrolyze 1 µ mole per minute at pH 8.3 and 25°C. 

Preparation: Two times crystallized from the euglobin fraction of porcine pancreas. Does not contain trypsin or chymotrypsin. 

Description: Hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. 

Inhibitors: DFP, elastinal, α-2-macroglobulin.

Specifications

Product Name

Elastase, CAS No.39445-21-1

Synonyms

pancreatic elastase | pancreatic elastase I | pancreatopeptidase E | Elastase from hog pancreas | CELA1 | ELA1 | ELS1 | Elastase-1

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

Biochemical and Physiological Mechanisms

Serine proteases that hydrolyze many proteins in addition to elastin.

Bioactivity

≥50 U/mg protein

Accession #

P00772

Predicted molecular weight

25.5 kDa

CAS

39445-21-1

Enzyme Commission Number

EC 3.4.21.36

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Unit definition

One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8.

References

1. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao.  (2024)  Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo.  INDUSTRIAL CROPS AND PRODUCTS,      [PMID:] [10.1016/j.indcrop.2024.118716]