Eosin B - ≥90% , CAS No.548-24-3

CAS: 548-24-3 Cat. No.: E100232 Molecular Weight: 624.06 Beilstein Registry Number: 4121964 EC Number: 208-943-1 PubChem CID: 26697441

GRADE & PURITY ≥90%

Synonyms

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt Acid Red 91 | Eosin bluish | Eosin Scarlet | Saffrosine Imperial Red | C.I. 45400 | 4,5-Dibromo-2,7-dinitrofluorescein disodium salt

Storage

Room temperature

Shipped In

Normal

Overview

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.
Eosin B is an adsorption and fluorescent indicator in microscopy.

Specifications

Synonyms

4′, 5′-Dibromo-2′, 7′-dinitrofluorescein disodium salt Acid Red 91 | Eosin bluish | Eosin Scarlet | Saffrosine Imperial Red | C.I. 45400 | 4, 5-Dibromo-2, 7-dinitrofluorescein disodium salt

Specifications & Purity

≥90%

Biochemical and Physiological Mechanisms

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer’s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining

Storage

Room temperature

Shipped In

Normal

Purity

≥90%

Names and Identifiers

• Pubchem Sid: 488200811
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488200811
• Canonical Smiles: [Na+].[Na+].[O-][N+](=O)c1cc2c(c3ccccc3C(=O)[O-])c3cc([N+](=O)[O-])c([O-])c(Br)c3oc2c(Br)c1=O
• WGK Germany: 3
• PubChem CID: 26697441
• Molecular Weight: 624.06
• Beilstein: 4121964

Chemical and Physical Properties

• Solubility: Soluble in water (390 g/L at 20°C), and ethanol.
• Sensitivity: Moisture sensitive.

References

1. Xia Qiu, Ling Wang, Yinggui Zhu.  (2021)  Cathodic electrochemiluminescence behavior of Eosin B and its quenching mechanism by Cr3+.  JOURNAL OF ELECTROANALYTICAL CHEMISTRY,      [PMID:] [10.1016/j.jelechem.2021.115031]

2. Xiu-Fang Yan, Zeng-Ping Chen, Yin-Yin Cui, Yuan-Liang Hu, Ru-Qin Yu.  (2016)  Quantitative generalized ratiometric fluorescence spectroscopy for turbid media based on probe encapsulated by biologically localized embedding.  ANALYTICA CHIMICA ACTA,      [PMID:27126788] [10.1016/j.aca.2016.03.033]

3. Jingfeng Dong, Mei Wang, Pan Zhang, Songqiu Yang, Jianyong Liu, Xueqiang Li, Licheng Sun.  (2011)  Promoting Effect of Electrostatic Interaction between a Cobalt Catalyst and a Xanthene Dye on Visible-Light-Driven Electron Transfer and Hydrogen Production.  Journal of Physical Chemistry C,      [PMID:] [10.1021/jp2040778]

4. Xiaohui Kang, Lanpeng Zhang, Wei Jia, Liang Yang, Changlong Jiang.  (2024)  Visual Detection of Methylglyoxal in Multiple Scenarios via NIR-Excitable Reversible Ratiometric Fluorescent Hydrogel Sensor.  ANALYTICAL CHEMISTRY,      [PMID:39698803] [10.1021/acs.analchem.4c05178]

5. Arif Nawaz, Jalwa Afreeq, Azhar Iqbal, Nisar Ali, Adnan Khan, Haifa Zhai, Rayya Ahmed Al Balushi, Mohammad M. Al-Hinaai, Thuraya Al-Harthy.  (2025)  Carboxymethyl chitosan derived Nx-C integrated with adjacent redox half reactions (MnCoSnO3): A dynamic contender for wastewater decontamination.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40623569] [10.1016/j.ijbiomac.2025.145757]