4-Nitrophenyl phosphate di(tris) salt, CAS No.68189-42-4

CAS: 68189-42-4 Cat. No.: N755422 Molecular Weight: 461.36 EC Number: 269-198-6

GRADE & PURITY phosphatase substrate

Overview

4-Nitrophenyl phosphate di(tris) salt has been used:

• as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
• to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
• for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Specifications

Product Name

4-Nitrophenyl phosphate di(tris) salt, CAS No.68189-42-4

Specifications & Purity

phosphatase substrate

Biochemical and Physiological Mechanisms

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotomet

CAS

68189-42-4

Molecule Type

Small molecule

Storage and Shipping

Concentration

phosphatase substrate

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Organic phosphoric acids and derivatives
      • Subclass Phosphate esters
        • Level5 Aryl phosphates
          • Level6 Aryl phosphomonoesters
            • Level7 Phenyl phosphates

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Organic phosphoric acids and derivatives
• Subclass: Phosphate esters
• Intermediate Tree Nodes: Aryl phosphates - Aryl phosphomonoesters
• Direct Parent: Phenyl phosphates
• Alternative Parents: Nitrobenzenes Phenoxy compounds Nitroaromatic compounds 1,2-aminoalcohols Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Primary alcohols Organopnictogen compounds Organic oxides Monoalkylamines Hydrocarbon derivatives
• Molecular Framework: Not available
• Substituents: Phenyl phosphate - Nitrobenzene - Phenoxy compound - Nitroaromatic compound - Monocyclic benzene moiety - Benzenoid - 1,2-aminoalcohol - C-nitro compound - Organic nitro compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Organic oxide - Primary amine - Primary alcohol - Organooxygen compound - Organonitrogen compound - Organopnictogen compound - Organic oxygen compound - Alcohol - Primary aliphatic amine - Organic nitrogen compound - Amine - Hydrocarbon derivative - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as phenyl phosphates. These are aromatic organooxygen compounds containing a phosphate group, which is O-esterified with a phenyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y