α-L-Rhamnosidase Activity Assay Kit (p-Nitrophenol, Micro Method)

Cat. No.: L1527050

GRADE & PURITY BioReagent

Overview

α-L-Rhamnosidase (EC 3.2.1.40) is a hydrolytic enzyme capable of hydrolyzing flavonoid glycosides commonly found in the daily diet. It is widely distributed in organisms such as bacteria and fungi in nature and holds broad prospects for industrial applications. This kit employs p-nitrophenyl-α-L-rhamnopyranoside (PNPR) as the substrate, which is enzymatically hydrolyzed to release the yellow product p-nitrophenol (PNP). PNP exhibits a characteristic absorbance peak at 405 nm. By continuously monitoring the initial linear increase in OD₄₀₅ per unit time (ΔOD₄₀₅/min) in kinetic mode, the α-L-rhamnosidase activity in the sample can be precisely calculated.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice