AIF Mouse mAb - Primary antibody, specific to AIFM1, Mouse IgG2a

Cat. No.: Ab087780
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
IF/ICC, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2a
Clone Number
8H1-B10-A12
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab087780-10μl
2
$99.90
50μl
Ab087780-50μl
1
$259.90
100μl
Ab087780-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab087780-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human AIF Antibody, Monoclonal(8H1-B10-A12), could be used for WB, IF, ICC and so on .
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Specifications

Product Name
AIF Mouse mAb - Primary antibody, specific to AIFM1, Mouse IgG2a
Synonyms
AIF | AIFapoptosis-inducing factor 1, mitochondrial | AIFM1 | apoptosis-inducing factor, mitochondrion-associated, 1 | COXPD6 | MGC111425 | PDCD8 | PDCD8striatal apoptosis-inducing factor | programmed cell death 8 (apoptosis-inducing factor) | Programmed
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Mouse
Specificity
AIFM1
Immunogen
Recombinant protein of human AIF (AA 1-170)
Positive Control
WB: HeLa, Ramos, HepG2, MCF-7, Jurkat lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Mouse IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

AIF Mouse mAb (Ab087780) - Western Blot
All lanes: AIF Mouse mAb (Ab087780) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 24.0 s

AIF Mouse mAb (Ab087780) - IF/ICC
Immunocytochemistry analysis of AIF in HeLa using AIF Mouse mAb (Ab087780).

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
AIFM1 Tbio Apoptosis-inducing factor 1, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303224Certificate of AnalysisJan 22, 2026 Ab087780
ZJ24F0303225Certificate of AnalysisJan 22, 2026 Ab087780
ZJ26F0635953Certificate of AnalysisJan 22, 2026 Ab087780
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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