Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG

Cat. No.: Ab138040

GRADE & PURITY Validated, ExactAb™, High Performance, 1mg/mL

Application

ELISA, IHC, WB

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

HRP

Overview

Goat Anti-Mouse IgG H&L (HRP) , Secondary antibody, could be used for WB, IHC, ELISA and so on.

Application
WB: 1/5000-1/20000
IHC: 1/500-1/1000
ELISA: 1/5000-1/20000

Specifications

Product Name

Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG

Synonyms

Goat Anti-Mouse IgG | Goat Anti Mouse IgG | Goat Anti-Mouse IgG (H+L) Secondary Antibody, HRP | Goat Anti-Mouse IgG (H+L), HRP | Goat Anti-Mouse IgG-HRP | Goat Anti Mouse IgG-HRP | Goat Anti Mouse IgG Ab (H/L) -HRP | Goat Anti-Mouse IgG (H&L) secondary an

Specifications & Purity

ExactAb™, High Performance, Validated, 1mg/mL

Host Species

Goat

Host species

Goat

Application

WB, ELISA, IHC-P

Immunogen

Mouse IgG

Positive Control

WB: HeLa lysates; IHC: Human breast cancer tissue

Conjugation

HRP

Grade

ExactAb™, High Performance, Validated

Antibody Type

secondary antibody

Clonality

polyclonal antibodies

Isotype

IgG

Purification Method

Affinity-Purification

Source

Serum

Concentration

1mg/mL

Note

This product is about to be sold out and taken down. The replacement product is Ab156255; Ab179001

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Images

ELISA: Goat Anti-Mouse IgG H&L (HRP) (Ab138040)

Goat Anti-Mouse IgG H&L (HRP) (Ab138040) - ELISA
The wells were coated with beta Actin Mouse mAb and BSA at 1.0 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Mouse IgG H&L (HRP) (Ab138040) then added at different dilution ratio followed by incubation for 30 min.
The Goat Anti-Mouse IgG H&L (HRP) (Ab138040) demonstrates exceptional performance in ELISA due to its high sensitivity and low background, ensuring accurate and reliable detection of mouse IgG.

Application

• Application: Dilution info
• WB:

  1/5000 - 1/20000 ;

• IHC:

  1/500-1/1000 ;

References

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]

2. Yifan Liu, Bo Xu, Jiexin Zheng, Zihui Jin, Di Sun, Tianyu Lan, Qiyang Zhao, Yue He, Jing Li, Yongliang Cui, Yaohai Zhang.  (2025)  Preparation of hapten and monoclonal antibody of diosmin and establishment of enzyme-linked immunosorbent assay.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.115257]