Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG

Cat. No.: Ab179004

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:647nm, Em:665nm, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

Cy5

Overview

Application
IF: 1:1000
Flow: 1:100-1:2000

Specifications

Product Name

Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG

Synonyms

Goat Anti-Mouse IgG H&L Antibody (Cy5) | Anti-Mouse IgG H&L Antibody (Cy5) | Mouse IgG H&L Antibody (Cy5) | Cy5-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (Cy5) | Anti-Mouse IgG Antibody (Cy5) | Mouse IgG Antibody (Cy5) | Goat ant

Specifications & Purity

ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm, 1.0 mg/mL

Host species

Goat

Immunogen

Mouse IgG

Conjugation

Cy5

Excitation (nm)

Red Laser(633nm)

Emission(nm)

665nm

Ex/Em(nm)

Ex:647nm;Em:665nm

Grade

Azide Free, Em:665nm, Ex:647nm, ExactAb™, Validated

Isotype

Goat IgG

Purification Method

Antigen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for one year. Store in the dark. Avoid freeze/thaw cycles.

Images

IF: Goat Anti-Mouse IgG H&L (Cy5) (Ab179004)

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (Cy5) (Ab179004)

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Goat Anti-Mouse IgG H&L (Cy5) (Ab179004)

Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) - Flow Cytometry
Flow Cytometry analysis of SK-N-SH cells labelling CD56 (red) with CD56 Mouse mAb (Ab006503). Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/2700 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  1:1000;

• Flow Cytometry:

  1:100-1:2000 ;

References

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]