ALDH6A1 Mouse mAb - Primärer Antikörper, specific to ALDH6A1, Mouse IgG1

Cat. No.: Ab088099

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Maus

Reactive species

Maus, Mensch

Isotype

Mouse IgG1

Clone Number

E7S8

Conjugation

Unconjugated

Übersicht

Mouse anti Human ALDH6A1 Antibody, Monoclonal (E7S8), could be used for WB, IHC, ICC/IF and so on.

Specifications

Product Name

ALDH6A1 Mouse mAb - Primärer Antikörper, specific to ALDH6A1, Mouse IgG1

Synonyme

AI314632 antibody | Aldehyde dehydrogenase 6 family member A1 antibody | Aldehyde dehydrogenase family 6 member A1 antibody | Aldehyde dehydrogenase family 6, subfamily A1 antibody | ALDH6A1 antibody | Malonate semialdehyde dehydrogenase antibody | Malona

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.

Wirtsspezies

Maus

Spezifität

ALDH6A1

Immunogen

Recombinant fusion protein of human ALDH6A1.

Positive Kontrolle

WB: Mouse liver, LNCaP lysates. IHC: Human colon tissue. IF/ICC: MCF7 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 28.1 s

Western Blot: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 96.3 s

IF/ICC: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - IF/ICC
Immunofluorescent analysis of ALDH6A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALDH6A1 Mouse mAb (Ab088099) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

IHC: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - IHC
Immunohistochemical analysis of ALDH6A1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALDH6A1 Mouse mAb (Ab088099) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anwendung

• Anwendung: Dilution info
• ICC/IF:

  1/10-1/50 ;

• IHC:

  1/50-1/200;

• WB:

  1/500-1/1000 ;

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (ALDH6A1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.