alpha-2-3,6-sialidase (BiNanH2), CAS No.9001-67-6

CAS: 9001-67-6 Cat. No.: A1435450 EC Number: 232-624-6

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥4000 U/mg protein; Protein concentration: See COA

Accession #

Q9Y3R4,Q99519,Q9UQ49,Q8WWR8

Protein Tag

C-His

Bioactivity

≥4000 U/mg protein

Overview

Bα-2-3,6-sialidase (BiNanH2), E.C 3.2.1.18, is derived from Bifidobacterium infantis. This enzyme is a specific exo-sialidase that catalyzes the hydrolysis of α2,3- and α2,6-linked sialic acids.

Reaction Equation:

Instructions for Use

1.Add the substrate sequentially to the reaction system, supplement with 5 mM CaCl₂, adjust the pH to 5.0 and mix well, then add an appropriate amount of Sialidase isoenzyme S (0.1 μg enzyme can process 1 μg substrate within 1 hour).

2.Incubate the sample at 37 °C for 2–4 hours or overnight.

Notes

1.Add the substrate sequentially to the reaction system, then add 5 mM MnCl₂ to adjust the pH to 5.0, mix thoroughly, and add an appropriate amount of BiNanH2.

2.Incubate the sample at 37 °C for 2–4 hours or overnight.

Precautions

For your safety and health, please wear a lab coat and disposable gloves during handling. This product is for research use only!

Specifications

Product Name

alpha-2-3,6-sialidase (BiNanH2), CAS No.9001-67-6

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥4000 U/mg protein; Protein concentration: See COA

Bioactivity

≥4000 U/mg protein

Accession #

Q9Y3R4, Q99519, Q9UQ49, Q8WWR8

Predicted molecular weight

42 kDa

CAS

9001-67-6

Enzyme Commission Number

EC 3.2.1.18

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥4000 U/mg protein; Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme that catalyzes the generation of 1 μmol each of lactose and Neu5Ac from Neu5Acα2,6Galβ1,4Glc per minute at 37°C.

References

1. Qian Wang, Zichao Peng, Bin Gui, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He.  (2025)  Sialic acid serves as a functional receptor for grass carp reovirus.  PLoS Pathogens,  21  (9): (e1013481).  [PMID:40911632] [10.1371/journal.ppat.1013481]

2. Yifei Wang, Mengyi Zhang, Yimeng An, Lanshu Li, Hao Wu, Ziqi Cheng, Ling Pan, Chaoying Yang, Weijin Huang, Yansheng Geng, Chenyan Zhao.  (2025)  A Bioluminescent Imaging Mouse Model for Seasonal Influenza Virus Infection Based on a Pseudovirus System.  Viruses-Basel,  17  (5): (686).  [PMID:40431697] [10.3390/v17050686]